Turmeric Extract Rescues Ethanol-Induced Developmental Defect in the Zebrafish Model for Fetal Alcohol Spectrum Disorder (FASD).

Autor: Muralidharan P; Dept. of Biology, Indiana Univ.-Purdue Univ. Indianapolis, Indianapolis, Ind., 46202, U.S.A., Connors CT; Dept. of Biology, Indiana Univ.-Purdue Univ. Indianapolis, Indianapolis, Ind., 46202, U.S.A., Mohammed AS; Dept. of Biology, Indiana Univ.-Purdue Univ. Indianapolis, Indianapolis, Ind., 46202, U.S.A., Sarmah S; Dept. of Biology, Indiana Univ.-Purdue Univ. Indianapolis, Indianapolis, Ind., 46202, U.S.A., Marrs K; Dept. of Biology, Indiana Univ.-Purdue Univ. Indianapolis, Indianapolis, Ind., 46202, U.S.A., Marrs JA; Dept. of Biology, Indiana Univ.-Purdue Univ. Indianapolis, Indianapolis, Ind., 46202, U.S.A., Chism GW; Dept. of Biology, Indiana Univ.-Purdue Univ. Indianapolis, Indianapolis, Ind., 46202, U.S.A.
Jazyk: angličtina
Zdroj: Journal of food science [J Food Sci] 2017 Sep; Vol. 82 (9), pp. 2221-2225. Date of Electronic Publication: 2017 Aug 10.
DOI: 10.1111/1750-3841.13830
Abstrakt: Prenatal ethanol exposure causes the most frequent preventable birth disorder, fetal alcohol spectrum disorder (FASD). The effect of turmeric extracts in rescuing an ethanol-induced developmental defect using zebrafish as a model was determined. Ethanol-induced oxidative stress is one of the major mechanisms underlying FASD. We hypothesize that antioxidant inducing properties of turmeric may alleviate ethanol-induced defects. Curcuminoid content of the turmeric powder extract (5 mg/mL turmeric in ethanol) was determined by UPLC and found to contain Curcumin (124.1 ± 0.2 μg/mL), Desmethoxycurcumin (43.4 ± 0.1 μg/mL), and Bisdemethoxycurcumin (36.6 ± 0.1 μg/mL). Zebrafish embryos were treated with 100 mM (0.6% v/v) ethanol during gastrulation through organogenesis (2 to 48 h postfertilization (hpf)) and supplemented with turmeric extract to obtain total curcuminoid concentrations of 0, 1.16, 1.72, or 2.32 μM. Turmeric supplementation showed significant rescue of the body length at 72 hpf compared to ethanol-treated embryos. The mechanism underlying the rescue remains to be determined.
(© 2017 Institute of Food Technologists®.)
Databáze: MEDLINE
Externí odkaz: