| Autor: |
Noal CB; Universidade Federal de Santa Maria. Departamento de Microbiologia e Parasitologia, Santa Maria, Rio Grande do Sul, Brazil., Monteiro DU; Universidade Federal de Santa Maria. Departamento de Microbiologia e Parasitologia, Santa Maria, Rio Grande do Sul, Brazil., Brum TF; Universidade Federal de Santa Maria, Departamento de Farmácia Industrial, Santa Maria, Rio Grande do Sul, Brazil., Emmanouilidis J; Universidade Federal de Santa Maria. Departamento de Microbiologia e Parasitologia, Santa Maria, Rio Grande do Sul, Brazil., Zanette RA; Universidade Federal do Rio Grande do Sul, Programa de Pós-Graduação em Ciências Biológicas: Farmacologia e Terapêutica, Porto Alegre, Rio Grande do Sul, Brazil., Morel AF; Universidade Federal de Santa Maria, Departamento de Química Orgânica, Santa Maria, Rio Grande do Sul, Brazil., Stefanon EBC; Centro Universitário Franciscano, Laboratório de Farmacologia, Toxicologia e Botânica, Santa Maria, Rio Grande do Sul, Brazil., Frosi M; Centro Universitário Franciscano, Laboratório de Farmacologia, Toxicologia e Botânica, Santa Maria, Rio Grande do Sul, Brazil., la Rue ML; Universidade Federal de Santa Maria. Departamento de Microbiologia e Parasitologia, Santa Maria, Rio Grande do Sul, Brazil. |
| Abstrakt: |
Scolicidal agents are important in the treatment of cystic echinococcosis. This study evaluated the scolicidal activity of the plant Blepharocalyx salicifolius (H.B.K.) Berg against Echinococcus ortleppi protoscoleces. The parasite species was identified by amplifying a fragment of the gene cytochrome c oxidase subunit 1 (COX 1). B. salicifolius crude extract at concentrations of 100, 200, 300 and 400 mg/mL was analyzed at different times (5, 10, 15, 30, 45 and 60 min). N-butanol and ethyl acetate fractions (100 and 200 mg/ mL) were also analyzed at 5, 10, 15 and 30 min. Both fractions showed 100% scolicidal activity at the concentration of 200 mg/mL at 5 min. Gallic acid, identified as the major compound of the ethyl acetate fraction- was responsible for the observed scolicidal activity. The results showed that crude extract and fractions of B. salicifolius have scolicidal effect against E. ortleppi protoscoleces. |
