An ex vivo model to quantitatively analyze cell migration in tissue.

Autor: O'Leary CJ; Queensland Drug Discovery Initiative, Institute for Molecular Bioscience, Queensland Bioscience Precinct, The University of Queensland, Brisbane, Australia., Weston M; Department of Clinical and Experimental Epilepsy, UCL Institute of Neurology, University College London, Queen Square, London, WC1N 3BG, UK., McDermott KW; Graduate Entry Medical School, University of Limerick, Limerick, Ireland.
Jazyk: angličtina
Zdroj: Developmental dynamics : an official publication of the American Association of Anatomists [Dev Dyn] 2018 Jan; Vol. 247 (1), pp. 201-211. Date of Electronic Publication: 2017 Sep 18.
DOI: 10.1002/dvdy.24562
Abstrakt: Background: Within the developing central nervous system, the ability of cells to migrate throughout the tissue parenchyma to reach their target destination and undergo terminal differentiation is vital to normal central nervous system (CNS) development. To develop novel therapies to treat the injured CNS, it is essential that the migratory behavior of cell populations is understood. Many studies have examined the ability of individual neurons to migrate through the developing CNS, describing specific modes of migration including locomotion and somal translocation. Few studies have investigated the mass migration of large populations of neural progenitors, particularly in the developing the spinal cord. Here, we describe a method to robustly analyze large numbers of migrating cells using a co-culture assay.
Results: The ex vivo tissue model promotes the survival and differentiation of co-cultured progenitor cells. Using this assay, we demonstrate that migrating neuroepithelial progenitor cells display region specific migration patterns within the dorsal and ventral spinal cord at defined developmental time points.
Conclusions: The technique described here is a viable ex vivo model to quantitatively analyze cell migration and differentiation. We demonstrate the ability to detect changes in cell migration within distinct tissue region across tissue samples using the technique described here. Developmental Dynamics 247:201-211, 2018. © 2017 Wiley Periodicals, Inc.
(© 2017 Wiley Periodicals, Inc.)
Databáze: MEDLINE
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