Influence of Magnetic Microparticles Isolation on Adenine Homonucleotides Structure.

Autor: Kremplova M; Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, Brno CZ-613 00, Czech Republic. mkremplova@volny.cz., Fialova D; Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, Brno CZ-613 00, Czech Republic. dana.dospivova@seznam.cz., Nejdl L; Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, Brno CZ-613 00, Czech Republic. lukasnejdl@gmail.com., Hynek D; Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, Brno CZ-613 00, Czech Republic. d.hynek@email.cz.; Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, Brno CZ-616 00, Czech Republic. d.hynek@email.cz., Trnkova L; Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, Brno CZ-613 00, Czech Republic. libuse@chemi.muni.cz.; Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, Brno CZ-616 00, Czech Republic. libuse@chemi.muni.cz.; Department of Chemistry, Faculty of Science, Masaryk University, Kamenice 5, Brno CZ-625 00, Czech Republic. libuse@chemi.muni.cz., Hubalek J; Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, Brno CZ-613 00, Czech Republic. hubalek@feec.vutbr.cz.; Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, Brno CZ-616 00, Czech Republic. hubalek@feec.vutbr.cz., Kizek R; Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, Brno CZ-613 00, Czech Republic. kizek@sci.muni.cz.; Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, Brno CZ-616 00, Czech Republic. kizek@sci.muni.cz., Adam V; Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, Brno CZ-613 00, Czech Republic. vojtech.adam@mendelu.cz.; Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, Brno CZ-616 00, Czech Republic. vojtech.adam@mendelu.cz.
Jazyk: angličtina
Zdroj: Materials (Basel, Switzerland) [Materials (Basel)] 2014 Feb 25; Vol. 7 (3), pp. 1455-1472. Date of Electronic Publication: 2014 Feb 25.
DOI: 10.3390/ma7031455
Abstrakt: The electroactivity of purine and pyrimidine bases is the most important property of nucleic acids that is very useful for determining oligonucleotides using square wave voltammetry. This study was focused on the electrochemical behavior of adenine-containing oligonucleotides before and after their isolation using paramagnetic particles. Two peaks were detected-peak A related to the reduction of adenine base and another peak B involved in the interactions between individual adenine strands and contributes to the formation of various spatial structures. The influence of the number of adenine bases in the strand in the isolation process using paramagnetic particles was investigated too.
Databáze: MEDLINE
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