Fluorescent Functionalization across Quaternary Structure in a Virus-like Particle.

Autor:	Chen Z; Department of Chemistry and Biochemistry, ‡Department of Biological Sciences, and §School of Behavioral and Brain Sciences, University of Texas at Dallas , Richardson, Texas 75080, United States., Boyd SD; Department of Chemistry and Biochemistry, ‡Department of Biological Sciences, and §School of Behavioral and Brain Sciences, University of Texas at Dallas , Richardson, Texas 75080, United States., Calvo JS; Department of Chemistry and Biochemistry, ‡Department of Biological Sciences, and §School of Behavioral and Brain Sciences, University of Texas at Dallas , Richardson, Texas 75080, United States., Murray KW; Department of Chemistry and Biochemistry, ‡Department of Biological Sciences, and §School of Behavioral and Brain Sciences, University of Texas at Dallas , Richardson, Texas 75080, United States., Mejia GL; Department of Chemistry and Biochemistry, ‡Department of Biological Sciences, and §School of Behavioral and Brain Sciences, University of Texas at Dallas , Richardson, Texas 75080, United States., Benjamin CE; Department of Chemistry and Biochemistry, ‡Department of Biological Sciences, and §School of Behavioral and Brain Sciences, University of Texas at Dallas , Richardson, Texas 75080, United States., Welch RP; Department of Chemistry and Biochemistry, ‡Department of Biological Sciences, and §School of Behavioral and Brain Sciences, University of Texas at Dallas , Richardson, Texas 75080, United States., Winkler DD; Department of Chemistry and Biochemistry, ‡Department of Biological Sciences, and §School of Behavioral and Brain Sciences, University of Texas at Dallas , Richardson, Texas 75080, United States., Meloni G; Department of Chemistry and Biochemistry, ‡Department of Biological Sciences, and §School of Behavioral and Brain Sciences, University of Texas at Dallas , Richardson, Texas 75080, United States., D'Arcy S; Department of Chemistry and Biochemistry, ‡Department of Biological Sciences, and §School of Behavioral and Brain Sciences, University of Texas at Dallas , Richardson, Texas 75080, United States., Gassensmith JJ; Department of Chemistry and Biochemistry, ‡Department of Biological Sciences, and §School of Behavioral and Brain Sciences, University of Texas at Dallas , Richardson, Texas 75080, United States.
Jazyk:	angličtina
Zdroj:	Bioconjugate chemistry [Bioconjug Chem] 2017 Sep 20; Vol. 28 (9), pp. 2277-2283. Date of Electronic Publication: 2017 Aug 22.
DOI:	10.1021/acs.bioconjchem.7b00305
Abstrakt:	Proteinaceous nanomaterials and, in particular, virus-like particles (VLPs) have emerged as robust and uniform platforms that are seeing wider use in biomedical research. However, there are a limited number of bioconjugation reactions for functionalizing the capsids, and very few of those involve functionalization across the supramolecular quaternary structure of protein assemblies. In this work, we exploit the recently described dibromomaleimide moiety as part of a bioconjugation strategy on VLP Qβ to break and rebridge the exposed and structurally important disulfides in good yields. Not only was the stability of the quaternary structure retained after the reaction, but the newly functionalized particles also became brightly fluorescent and could be tracked in vitro using a commercially available filter set. Consequently, we show that this highly efficient bioconjugation reaction not only introduces a new functional handle "between" the disulfides of VLPs without compromising their thermal stability but also can be used to create a fluorescent probe.
Databáze:	MEDLINE
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