Janus dendrimersomes coassembled from fluorinated, hydrogenated, and hybrid Janus dendrimers as models for cell fusion and fission.

Autor:	Xiao Q; Roy & Diana Vagelos Laboratories, Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104-6323., Sherman SE; Roy & Diana Vagelos Laboratories, Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104-6323., Wilner SE; Roy & Diana Vagelos Laboratories, Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104-6323., Zhou X; Roy & Diana Vagelos Laboratories, Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104-6323., Dazen C; Roy & Diana Vagelos Laboratories, Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104-6323., Baumgart T; Roy & Diana Vagelos Laboratories, Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104-6323., Reed EH; Department of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104-6321., Hammer DA; Department of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104-6321.; Department of Chemical and Biomolecular Engineering, University of Pennsylvania, Philadelphia, PA 19104-6391., Shinoda W; Department of Materials Chemistry, Nagoya University, Nagoya 464-8603, Japan., Klein ML; Institute of Computational Molecular Science, Temple University, Philadelphia, PA 19122 mlklein@temple.edu percec@sas.upenn.edu., Percec V; Roy & Diana Vagelos Laboratories, Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104-6323; mlklein@temple.edu percec@sas.upenn.edu.
Jazyk:	angličtina
Zdroj:	Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2017 Aug 22; Vol. 114 (34), pp. E7045-E7053. Date of Electronic Publication: 2017 Aug 07.
DOI:	10.1073/pnas.1708380114
Abstrakt:	A three-component system of Janus dendrimers (JDs) including hydrogenated, fluorinated, and hybrid hydrogenated-fluorinated JDs are reported to coassemble by film hydration at specific ratios into an unprecedented class of supramolecular Janus particles (JPs) denoted Janus dendrimersomes (JDSs). They consist of a dumbbell-shaped structure composed of an onion-like hydrogenated vesicle and an onion-like fluorinated vesicle tethered together. The synthesis of dye-tagged analogs of each JD component enabled characterization of JDS architectures with confocal fluorescence microscopy. Additionally, a simple injection method was used to prepare submicron JDSs, which were imaged with cryogenic transmission electron microscopy (cryo-TEM). As reported previously, different ratios of the same three-component system yielded a variety of structures including homogenous onion-like vesicles, core-shell structures, and completely self-sorted hydrogenated and fluorinated vesicles. Taken together with the JDSs reported herein, a self-sorting pathway is revealed as a function of the relative concentration of the hybrid JD, which may serve to stabilize the interface between hydrogenated and fluorinated bilayers. The fission-like pathway suggests the possibility of fusion and fission processes in biological systems that do not require the assistance of proteins but instead may result from alterations in the ratios of membrane composition. Competing Interests: Conflict of interest statement: D.A.T. has an adjunct appointment at the University of Pennsylvania. It is not his primary affiliation.
Databáze:	MEDLINE
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