Investigating the race for the surface and skin integration in clinically retrieved abutments with two-photon microscopy.

Autor: Kapsokalyvas D; Department of Molecular Cell Biology, CARIM School for Cardiovascular diseases, Maastricht University Medical Center, UNS 50, 6229 ER Maastricht, The Netherlands., van Hoof M; Department of Otorhinolaryngology and Head and Neck Surgery, Maastricht University Medical Center, PO Box 5800, 6202, AZ, Maastricht, The Netherlands. Electronic address: marc.hoofvan@mumc.nl., Wigren S; Cochlear Bone Anchored Solutions, AB, Mölnlycke, Sweden., Chimhanda T; Department of Molecular Cell Biology, CARIM School for Cardiovascular diseases, Maastricht University Medical Center, UNS 50, 6229 ER Maastricht, The Netherlands., Kuijpers HJ; Department of Molecular Cell Biology, CARIM School for Cardiovascular diseases, Maastricht University Medical Center, UNS 50, 6229 ER Maastricht, The Netherlands., Ramaekers FCS; Department of Molecular Cell Biology, GROW-School for Oncology and Developmental Biology, Maastricht University Medical Center, PO Box 616, 6200 MD Maastricht, The Netherlands., Stokroos RJ; Department of Otorhinolaryngology and Head and Neck Surgery, Maastricht University Medical Center, PO Box 5800, 6202, AZ, Maastricht, The Netherlands., van Zandvoort MAMJ; Department of Molecular Cell Biology, CARIM School for Cardiovascular diseases, Maastricht University Medical Center, UNS 50, 6229 ER Maastricht, The Netherlands.
Jazyk: angličtina
Zdroj: Colloids and surfaces. B, Biointerfaces [Colloids Surf B Biointerfaces] 2017 Nov 01; Vol. 159, pp. 97-107. Date of Electronic Publication: 2017 Jul 27.
DOI: 10.1016/j.colsurfb.2017.07.072
Abstrakt: Bone conduction hearing implants can rehabilitate some types of hearing loss. A hydroxyapatite (HA)-coated skin-penetrating abutment was developed to allow for soft tissue preservation and increased skin-abutment adherence. Inflammation is thought to relate to bacterial infection of pockets around the abutment. Upon integration, the host's ability to cover the abutment surface ("race for the surface"), and thus control and prevent competitive bacteria from colonizing it, is improved. However, the attachment mechanisms behind it are not clear. In this study, we applied two-photon microscopy to visualize tissue attachment on abutments retrieved from patients. Skin integration markers were validated and applied to four HA-coated abutments. Evidence of skin integration was found, including the presence of hemidesmosomes, a basement membrane, dermal collagen and vascularization. Cases with clinical signs of severe inflammation and evident biofilm formation showed limited skin integration based on these indicators, confirming the applicability of the "race for the surface" model.
(Copyright © 2017 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: