DACH1 stimulates shear stress-guided endothelial cell migration and coronary artery growth through the CXCL12-CXCR4 signaling axis.

Autor:	Chang AH; Department of Developmental Biology, Stanford University School of Medicine, Stanford, California 94305, USA.; Department of Biology, Stanford University, Stanford, California 94305, USA., Raftrey BC; Department of Biology, Stanford University, Stanford, California 94305, USA., D'Amato G; Department of Biology, Stanford University, Stanford, California 94305, USA., Surya VN; Department of Chemical Engineering, Stanford University, Stanford, California 94305, USA., Poduri A; Department of Biology, Stanford University, Stanford, California 94305, USA., Chen HI; Department of Developmental Biology, Stanford University School of Medicine, Stanford, California 94305, USA.; Department of Biology, Stanford University, Stanford, California 94305, USA., Goldstone AB; Department of Cardiothoracic Surgery, Stanford University School of Medicine, Stanford, California 94305, USA.; Department of Health Research and Policy-Epidemiology, Stanford University School of Medicine, Stanford, California 94305, USA., Woo J; Department of Cardiothoracic Surgery, Stanford University School of Medicine, Stanford, California 94305, USA., Fuller GG; Department of Chemical Engineering, Stanford University, Stanford, California 94305, USA., Dunn AR; Department of Chemical Engineering, Stanford University, Stanford, California 94305, USA., Red-Horse K; Department of Biology, Stanford University, Stanford, California 94305, USA.
Jazyk:	angličtina
Zdroj:	Genes & development [Genes Dev] 2017 Jul 01; Vol. 31 (13), pp. 1308-1324. Date of Electronic Publication: 2017 Aug 04.
DOI:	10.1101/gad.301549.117
Abstrakt:	Sufficient blood flow to tissues relies on arterial blood vessels, but the mechanisms regulating their development are poorly understood. Many arteries, including coronary arteries of the heart, form through remodeling of an immature vascular plexus in a process triggered and shaped by blood flow. However, little is known about how cues from fluid shear stress are translated into responses that pattern artery development. Here, we show that mice lacking endothelial Dach1 had small coronary arteries, decreased endothelial cell polarization, and reduced expression of the chemokine Cxcl12 Under shear stress in culture, Dach1 overexpression stimulated endothelial cell polarization and migration against flow, which was reversed upon CXCL12/CXCR4 inhibition. In vivo, DACH1 was expressed during early arteriogenesis but was down in mature arteries. Mature artery-type shear stress (high, uniform laminar) specifically down-regulated DACH1, while the remodeling artery-type flow (low, variable) maintained DACH1 expression. Together, our data support a model in which DACH1 stimulates coronary artery growth by activating Cxcl12 expression and endothelial cell migration against blood flow into developing arteries. This activity is suppressed once arteries reach a mature morphology and acquire high, laminar flow that down-regulates DACH1. Thus, we identified a mechanism by which blood flow quality balances artery growth and maturation. (© 2017 Chang et al.; Published by Cold Spring Harbor Laboratory Press.)
Databáze:	MEDLINE
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