Validation of a Novel Diagnostic Kit Using the Semidry Dot-Blot Method to Detect Metastatic Lymph Nodes in Breast Cancer: Distinguishing Macrometastases From Nonmacrometastases.

Autor: Otsubo R; Division of Surgical Oncology, Nagasaki University Hospital, Nagasaki, Japan. Electronic address: rotsubo@nagasaki-u.ac.jp., Hirakawa H; Department of Gynecology, Aiyuukai Memorial Hospital, Chiba, Japan., Oikawa M; Division of Surgical Oncology, Nagasaki University Hospital, Nagasaki, Japan; Division of Breast Surgery, New-wa-kai Oikawa Hospital, Fukuoka, Japan., Baba M; Division of Surgical Oncology, Nagasaki University Hospital, Nagasaki, Japan., Inamasu E; Division of Surgical Oncology, Nagasaki University Hospital, Nagasaki, Japan., Shibata K; Department of Surgery, The Japanese Red Cross Nagasaki Genbaku Hospital, Nagasaki, Japan., Hatachi T; Division of Surgical Oncology, Nagasaki University Hospital, Nagasaki, Japan., Matsumoto M; Division of Surgical Oncology, Nagasaki University Hospital, Nagasaki, Japan., Yano H; Division of Surgical Oncology, Nagasaki University Hospital, Nagasaki, Japan., Abe K; Department of Pathology, Nagasaki University Hospital, Nagasaki, Japan., Taniguchi H; Department of Surgery, The Japanese Red Cross Nagasaki Genbaku Hospital, Nagasaki, Japan., Nakashima M; Department of Tumor and Diagnostic Pathology, Atomic Bomb Disease Institute, Nagasaki University, Nagasaki, Japan., Nagayasu T; Division of Surgical Oncology, Nagasaki University Hospital, Nagasaki, Japan.
Jazyk: angličtina
Zdroj: Clinical breast cancer [Clin Breast Cancer] 2018 Jun; Vol. 18 (3), pp. e345-e351. Date of Electronic Publication: 2017 Jul 15.
DOI: 10.1016/j.clbc.2017.07.009
Abstrakt: Background: The semidry dot-blot method is a diagnostic procedure for detecting lymph node (LN) metastases using the presence of cytokeratin (CK) in lavage fluid from sectioned LNs. We evaluated 2 novel kits that use newly developed anti-CK-19 antibodies to diagnose LN metastases in breast cancer.
Patients and Methods: We examined 159 LNs dissected that we sliced at 2-mm intervals and washed with phosphate-buffered saline. The suspended cells in the lavage were centrifuged and lysed to extract protein. This extracted protein was used with a low-power and a high-power kit to diagnose LN metastasis. Diagnoses on the basis of the kits were compared with pathological diagnoses.
Results: Of the 159 LNs, 68 were assessed as positive and 91 as negative in permanent section examination. Sensitivity, specificity, and accuracy of the low-power kit for detecting LN metastases was 83.8%, 100%, and 93.1%, respectively. Those of the high-power kit were 92.6%, 92.3%, and 92.5%, respectively. Combining the low- and high-power kit results, those for distinguishing macrometastases were 94.5%, 95.2%, and 95.0%, respectively. Diagnosis was achieved in approximately 20 minutes, at a cost of less than $30 USD.
Conclusion: The kits were accurate, fast, and cost-effective in diagnosing LN metastases without the loss of LN tissue.
(Copyright © 2017 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: