| Autor: |
Deng Y; Stockbridge School of Agriculture, University of Massachusetts Amherst , 410 Paige Lab, Amherst, Massachusetts 01003, United States., Petersen EJ; Biosystems and Biomaterials Division, National Institute of Standards and Technology , 100 Bureau Drive, Building 227 Room A222, Gaithersburg, Maryland 20899, United States., Challis KE; Department of Chemistry, Colorado School of Mines , Golden, Colorado 80401, United States., Rabb SA; Chemical Sciences Division, National Institute of Standards and Technology , Gaithersburg, Maryland 20899, United States., Holbrook RD; Materials Measurement Science Division, National Institute of Standards and Technology , Gaithersburg, Maryland 20899, United States., Ranville JF; Department of Chemistry, Colorado School of Mines , Golden, Colorado 80401, United States., Nelson BC; Biosystems and Biomaterials Division, National Institute of Standards and Technology , 100 Bureau Drive, Building 227 Room A222, Gaithersburg, Maryland 20899, United States., Xing B; Stockbridge School of Agriculture, University of Massachusetts Amherst , 410 Paige Lab, Amherst, Massachusetts 01003, United States. |
| Abstrakt: |
Understanding the translocation of nanoparticles (NPs) into plants is challenging because qualitative and quantitative methods are still being developed and the comparability of results among different methods is unclear. In this study, uptake of titanium dioxide NPs and larger bulk particles (BPs) in rice plant (Oryza sativa L.) tissues was evaluated using three orthogonal techniques: electron microscopy, single-particle inductively coupled plasma mass spectroscopy (spICP-MS) with two different plant digestion approaches, and total elemental analysis using ICP optical emission spectroscopy. In agreement with electron microscopy results, total elemental analysis of plants exposed to TiO 2 NPs and BPs at 5 and 50 mg/L concentrations revealed that TiO 2 NPs penetrated into the plant root and resulted in Ti accumulation in above ground tissues at a higher level compared to BPs. spICP-MS analyses revealed that the size distributions of internalized particles differed between the NPs and BPs with the NPs showing a distribution with smaller particles. Acid digestion resulted in higher particle numbers and the detection of a broader range of particle sizes than the enzymatic digestion approach, highlighting the need for development of robust plant digestion procedures for NP analysis. Overall, there was agreement among the three techniques regarding NP and BP penetration into rice plant roots and spICP-MS showed its unique contribution to provide size distribution information. |
