Microphysiological Systems to Assess Nonclinical Toxicity.

Autor: Van Ness KP; Department of Pharmaceutics, University of Washington, Seattle, Washington., Chang SY; Department of Occupational and Environmental Health Sciences, University of Washington, Seattle, Washington., Weber EJ; Department of Pharmaceutics, University of Washington, Seattle, Washington., Zumpano D; Nortis, Inc. Seattle, Washington., Eaton DL; Department of Occupational and Environmental Health Sciences, University of Washington, Seattle, Washington., Kelly EJ; Department of Pharmaceutics, University of Washington, Seattle, Washington.
Jazyk: angličtina
Zdroj: Current protocols in toxicology [Curr Protoc Toxicol] 2017 Aug 04; Vol. 73, pp. 14.18.1-14.18.28. Date of Electronic Publication: 2017 Aug 04.
DOI: 10.1002/cptx.27
Abstrakt: The liver and the kidney are key toxicity target organs during drug development campaigns, as they typically carry the burden of drug transport and metabolism. Primary hepatocytes and proximal tubule epithelial cells grown in traditional in vitro 2-D culture systems do not maintain transporter and metabolic functions, thus limiting their utility for nonclinical toxicology investigations. We have developed a renal and hepatic microphysiological system (MPS) platform that uses a commercially available MPS device as the core cell culture platform for our methodologies. We describe protocols for isolating and propagating human proximal epithelial cells and how to seed and culture a renal MPS to recapitulate the human proximal tubule. We present two methods to culture hepatocytes within an MPS and the steps required to connect a renal MPS to a liver MPS. © 2017 by John Wiley & Sons, Inc.
(Copyright © 2017 John Wiley & Sons, Inc.)
Databáze: MEDLINE
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