| Autor: |
Anderson RR; a Departamento de Ciências Exatas e da Terra , Universidade Federal de São Paulo , Diadema , Brazil., Girola N; b Departamento de Microbiologia, Imunologia e Parasitologia , Universidade Federal de São Paulo , São Paulo , Brazil., Figueiredo CR; b Departamento de Microbiologia, Imunologia e Parasitologia , Universidade Federal de São Paulo , São Paulo , Brazil., Londero VS; a Departamento de Ciências Exatas e da Terra , Universidade Federal de São Paulo , Diadema , Brazil., Lago JHG; c Centro de Ciências Naturais e Humanas , Universidade Federal do ABC , Santo André , Brazil. |
| Jazyk: |
angličtina |
| Zdroj: |
Natural product research [Nat Prod Res] 2018 Apr; Vol. 32 (7), pp. 859-862. Date of Electronic Publication: 2017 Aug 03. |
| DOI: |
10.1080/14786419.2017.1361952 |
| Abstrakt: |
Aiming detection of circadian variation in the chemical composition of volatiles from Piper regnellii, the leaves were collected during four different periods (8, 12, 16 and 20 h) in the same day. After extraction by hydrodistillation and GC/MS analysis, no significant variation was observed for the main compounds: germacrene D (45.6 ± 1.5-51.4 ± 3.1%), α-chamigrene (8.9 ± 1.3-11.3 ± 2.7%) and β-caryophyllene (8.2 ± 0.9-9.5 ± 0.3%). Evaluation of in vitro cytotoxicity against several cancer and non-tumourigenic cells indicated promising activity, especially to HeLa (human cervical carcinoma) with IC 50 ranging from 11 ± 3 to 17 ± 3 μg/mL. The obtained volatile oils were pooled and subjected to fractionation to afford pure β-caryophyllene, α-chamigrene and germacrene D, being this last compound the more active against HeLa cells with IC 50 of 7 ± 1 μg/mL (34 ± 5 μM). Therefore, the predominance of germacrene D in all analysed oils could justify, at least in part, the activity observed for the volatile compounds from P. regnellii leaves. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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