Interplay between Lactobacillus rhamnosus GG and Candida and the involvement of exopolysaccharides.

Autor: Allonsius CN; Department of Bioscience Engineering, Research Group Environmental Ecology and Applied Microbiology, University of Antwerp, Groenenborgerlaan 171, B-2020, Antwerp, Belgium., van den Broek MFL; Department of Bioscience Engineering, Research Group Environmental Ecology and Applied Microbiology, University of Antwerp, Groenenborgerlaan 171, B-2020, Antwerp, Belgium., De Boeck I; Department of Bioscience Engineering, Research Group Environmental Ecology and Applied Microbiology, University of Antwerp, Groenenborgerlaan 171, B-2020, Antwerp, Belgium., Kiekens S; Department of Bioscience Engineering, Research Group Environmental Ecology and Applied Microbiology, University of Antwerp, Groenenborgerlaan 171, B-2020, Antwerp, Belgium.; Department of Pharmaceutical, biomedical and veterinary sciences, Laboratory of Pharmaceutical Technology and Biopharmacy, University of Antwerp, Wilrijk, Belgium., Oerlemans EFM; Department of Bioscience Engineering, Research Group Environmental Ecology and Applied Microbiology, University of Antwerp, Groenenborgerlaan 171, B-2020, Antwerp, Belgium., Kiekens F; Department of Pharmaceutical, biomedical and veterinary sciences, Laboratory of Pharmaceutical Technology and Biopharmacy, University of Antwerp, Wilrijk, Belgium., Foubert K; Department of Pharmaceutical Sciences, Natural Products & Food Research and Analysis, University of Antwerp, Wilrijk, Belgium., Vandenheuvel D; Department of Bioscience Engineering, Research Group Environmental Ecology and Applied Microbiology, University of Antwerp, Groenenborgerlaan 171, B-2020, Antwerp, Belgium., Cos P; Department of Biomedical Sciences, Laboratory of Microbiology, Parasitology and Hygiene, University of Antwerp, Wilrijk, Belgium., Delputte P; Department of Biomedical Sciences, Laboratory of Microbiology, Parasitology and Hygiene, University of Antwerp, Wilrijk, Belgium., Lebeer S; Department of Bioscience Engineering, Research Group Environmental Ecology and Applied Microbiology, University of Antwerp, Groenenborgerlaan 171, B-2020, Antwerp, Belgium.
Jazyk: angličtina
Zdroj: Microbial biotechnology [Microb Biotechnol] 2017 Nov; Vol. 10 (6), pp. 1753-1763. Date of Electronic Publication: 2017 Aug 03.
DOI: 10.1111/1751-7915.12799
Abstrakt: A number of clinical studies have shown protective effects of lactobacilli against Candida species in the gastrointestinal tract, the urogenital tract and the oral cavity, while others did not show clear effects. Evidence on the mode of action of lactobacilli against Candida is also still lacking. In this study, the anti-Candida activity of the model probiotic strain Lactobacillus rhamnosus GG was explored in different assays to determine molecular interactions. We found that L. rhamnosus GG was able to interfere with Candida growth, morphogenesis and adhesion. These three aspects of Candida's physiology are all crucial to its opportunistic pathogenesis. In follow-up assays, we compared the activity of L. rhamnosus GG wild-type with its exopolysaccharide (EPS)-deficient mutant and purified EPS to evaluate the involvement of this outer carbohydrate layer. Our data demonstrate that purified EPS can both interfere with hyphal formation and adhesion to epithelial cells, which indicates that EPS is part of a combined molecular mechanism underlying the antihyphal and anti-adhesion mechanisms of L. rhamnosus GG.
(© 2017 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology.)
Databáze: MEDLINE
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