| Autor: |
Shormanov VK; Kursk State Medical University, Ministry of Health of the Russia, Kursk, Russia, 305041., Tsatsua EP; Kursk State Medical University, Ministry of Health of the Russia, Kursk, Russia, 305041., Astashkina AP; Institute of Natural Resources, Tomsk National Research Polytechnical University, Tomsk, Russia, 634050. |
| Jazyk: |
ruština |
| Zdroj: |
Sudebno-meditsinskaia ekspertiza [Sud Med Ekspert] 2017; Vol. 60 (4), pp. 34-39. |
| DOI: |
10.17116/sudmed201760434-39 |
| Abstrakt: |
The objective of this work was to study peculiarities of identification of 2,4-di-tert-butyl hydroxybenzene (2,4-DTBHOB) in blood and the tissues of various organs with the use TLC, UR- and UV-spectrophotometry. The results of the study suggest the expediency of the application of ethylacetate as the extractive agent for the isolation of 2,4-DTBHOB from the biological materials. The optimal conditions for 2,4-DTBHOB extraction by this method were developed based on the decontamination of the analyte by means of removal of the organic substances of biological matrices using the L 40/100 mcm sorbent columns with the hexan/dioxane mixture (8.5:1.5) as the mobile phase. The method for the determination of 2,4-di-tert-butyl hydroxybenzene in the tissues of various organs (namely, liver) and blood has been developed. The proposed method allows to determine the minimal quantities of 2,4-DTBHOB present in the biological objects equivalent to 0.36 mg and 0.28 mg in the hepatic tissue and blood respectively. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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