| Autor: |
Castro MG; Instituto Multidisciplinario de Investigaciones Biológicas San Luis (IMIBIO-SL) CONICET and Universidad Nacional de San Luis, San Luis, Argentina., Campos LE; Instituto Multidisciplinario de Investigaciones Biológicas San Luis (IMIBIO-SL) CONICET and Universidad Nacional de San Luis, San Luis, Argentina., Rodriguez YI; Instituto Multidisciplinario de Investigaciones Biológicas San Luis (IMIBIO-SL) CONICET and Universidad Nacional de San Luis, San Luis, Argentina., Alvarez SE; Instituto Multidisciplinario de Investigaciones Biológicas San Luis (IMIBIO-SL) CONICET and Universidad Nacional de San Luis, San Luis, Argentina. sealvarez98@gmail.com. |
| Jazyk: |
angličtina |
| Zdroj: |
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2018; Vol. 1697, pp. 117-131. |
| DOI: |
10.1007/7651_2017_51 |
| Abstrakt: |
Sphingosine-1-phosphate (S1P) is a bioactive lipid that modulates migratory behavior of cells during embryonic development. In addition, S1P might promote tumor progression by enhancing migratory ability and invasiveness of tumor cells. Migration is a complex process that implies cytoskeletal reorganization and formation of structures that enable cell movement. Besides having similar requirements than migration, invasion also involves proteolytic degradation of extracellular matrix (ECM). Matrix metalloproteases (MMPs) have been identified to break down components of the ECM, allowing cancer cells to spread out of the primary tumor. In this chapter, we will describe different techniques to study migration and invasion induced by S1P. To this end, we include detailed protocols of end-point assays to study migration/invasion, and zymography assay to analyze MMP-2 and MMP-9 activity that were standardized in our laboratory in human melanoma cell lines. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
