Rapid expression and purification of the hepatitis delta virus antigen using the methylotropic yeast Pichia pastoris.
| Autor: | Cartwright SP; School of Life & Health Sciences, Aston University, Aston Triangle, Birmingham, B4 7ET, UK., Bill RM; School of Life & Health Sciences, Aston University, Aston Triangle, Birmingham, B4 7ET, UK., Sy BT; Department of Molecular Biology, 108 Military Central Hospital, 1 Tran Thanh Tong, Ha Noi City, Viet Nam., Tran-Van H; Faculty of Biology and Biotechnology, University of Science, Vietnam National University, Ho Chi Minh City, Viet Nam., Nguyen HM; Center for Molecular Biology, Institute of Research and Development, Duy Tan University, K7/25 Quang Trung, Da Nang City, Viet Nam. hungmolbio@gmail.com. |
|---|---|
| Jazyk: | angličtina |
| Zdroj: | BMC research notes [BMC Res Notes] 2017 Jul 27; Vol. 10 (1), pp. 340. Date of Electronic Publication: 2017 Jul 27. |
| DOI: | 10.1186/s13104-017-2692-8 |
| Abstrakt: | Objective: Patients with dual hepatitis B (HBV) and hepatitis D (HDV) virus infection are at an increased risk of progression to liver cirrhosis and hepatocellular carcinoma than patients with a single viral infection. Treatment of viral hepatitis due to dual HBV/HDV infection represents a challenge. Currently there is no vaccine against HDV. Recombinant production of HDV antigen (HDAg) is the first step towards a potential vaccine candidate and the development of assays for HDV detection. Results: This study demonstrates the expression of one HDAg isoform, S-HDAg, in Pichia pastoris. A recombinant vector carrying a tagged gene encoding S-HDAg under the control of the methanol-inducible promoter AOX1 was designed and integrated into P. pastoris X33. The protein, which was purified using a Ni 2+ affinity column and eluted at 100-150 mM imidazole, has potential as a recombinant antigen for further study. |
| Databáze: | MEDLINE |
| Externí odkaz: |
|