Fast two-photon imaging of subcellular voltage dynamics in neuronal tissue with genetically encoded indicators.

Autor: Chamberland S; Department of Psychiatry and Neuroscience, Quebec Mental Health Institute, Université Laval, Québec, Canada., Yang HH; Department of Neurobiology, Stanford University, Stanford, United States., Pan MM; Department of Bioengineering, Stanford University, Stanford, United States.; Department of Pediatrics, Stanford University, Stanford, United States., Evans SW; Department of Neurobiology, Stanford University, Stanford, United States.; Department of Bioengineering, Stanford University, Stanford, United States.; Department of Pediatrics, Stanford University, Stanford, United States., Guan S; Department of Neuroscience, Baylor College of Medicine, Houston, United States., Chavarha M; Department of Neurobiology, Stanford University, Stanford, United States.; Department of Bioengineering, Stanford University, Stanford, United States.; Department of Pediatrics, Stanford University, Stanford, United States., Yang Y; Department of Neurobiology, Stanford University, Stanford, United States.; Department of Bioengineering, Stanford University, Stanford, United States.; Department of Pediatrics, Stanford University, Stanford, United States., Salesse C; Department of Psychiatry and Neuroscience, Quebec Mental Health Institute, Université Laval, Québec, Canada., Wu H; Stanford Cardiovascular Institute, Stanford University, Stanford, United States., Wu JC; Stanford Cardiovascular Institute, Stanford University, Stanford, United States., Clandinin TR; Department of Neurobiology, Stanford University, Stanford, United States., Toth K; Department of Psychiatry and Neuroscience, Quebec Mental Health Institute, Université Laval, Québec, Canada., Lin MZ; Department of Neurobiology, Stanford University, Stanford, United States.; Department of Bioengineering, Stanford University, Stanford, United States.; Department of Pediatrics, Stanford University, Stanford, United States., St-Pierre F; Department of Bioengineering, Stanford University, Stanford, United States.; Department of Pediatrics, Stanford University, Stanford, United States.; Department of Neuroscience, Baylor College of Medicine, Houston, United States.
Jazyk: angličtina
Zdroj: ELife [Elife] 2017 Jul 27; Vol. 6. Date of Electronic Publication: 2017 Jul 27.
DOI: 10.7554/eLife.25690
Abstrakt: Monitoring voltage dynamics in defined neurons deep in the brain is critical for unraveling the function of neuronal circuits but is challenging due to the limited performance of existing tools. In particular, while genetically encoded voltage indicators have shown promise for optical detection of voltage transients, many indicators exhibit low sensitivity when imaged under two-photon illumination. Previous studies thus fell short of visualizing voltage dynamics in individual neurons in single trials. Here, we report ASAP2s, a novel voltage indicator with improved sensitivity. By imaging ASAP2s using random-access multi-photon microscopy, we demonstrate robust single-trial detection of action potentials in organotypic slice cultures. We also show that ASAP2s enables two-photon imaging of graded potentials in organotypic slice cultures and in Drosophila . These results demonstrate that the combination of ASAP2s and fast two-photon imaging methods enables detection of neural electrical activity with subcellular spatial resolution and millisecond-timescale precision.
Databáze: MEDLINE
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