| Autor: |
da Rocha DG; Laboratório de Biologia do Reconhecer (LBR), Centro de Biociências e Biotecnologia (CBB), Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Alberto Lamego Avenue, 2000 - Campos dos Goytacazes, Postal Code 28013-602 Rio de Janeiro, Brazil., Fernandez JH; Laboratório de Química e Função de Proteínas e Peptídeos (LQFPP), Centro de Biociências e Biotecnologia (CBB), Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Alberto Lamego Avenue, 2000 - Campos dos Goytacazes, Postal Code 28013-602 Rio de Janeiro, Brazil., de Almeida CMC; Laboratório de Sanidade Animal (LSA), Centro de Ciências e Tecnologias Agropecuárias (CCTA), Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Alberto Lamego Avenue, 2000 - Campos dos Goytacazes, Postal Code 28013-602 Rio de Janeiro, Brazil., da Silva CL; Laboratório de Biologia do Reconhecer (LBR), Centro de Biociências e Biotecnologia (CBB), Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Alberto Lamego Avenue, 2000 - Campos dos Goytacazes, Postal Code 28013-602 Rio de Janeiro, Brazil., Magnoli FC; Laboratório de Imunoquímica, Instituto Butantan, Vital Brazil Avenue, 1500 - City of São Paulo, Postal Code 05503-900 São Paulo, Brazil., da Silva OÉ; Laboratório de Imunoquímica, Instituto Butantan, Vital Brazil Avenue, 1500 - City of São Paulo, Postal Code 05503-900 São Paulo, Brazil., da Silva WD; Laboratório de Imunoquímica, Instituto Butantan, Vital Brazil Avenue, 1500 - City of São Paulo, Postal Code 05503-900 São Paulo, Brazil. |
| Abstrakt: |
The data presented in this article are related to the research article entitled "Development of IgY antibodies against anti-snake toxins endowed with highly lethal neutralizing activity" (da Rocha et al., 2017) [1]. Complementarity-determining region (CDR) sequences are variable antibody (Ab) sequences that respond with specificity, duration and strength to identify and bind to antigen (Ag) epitopes. B lymphocytes isolated from hens immunized with Bitis arietans (Ba) and anti- Crotalus durissus terrificus (Cdt) venoms and expressing high specificity, affinity and toxicity neutralizing antibody titers were used as DNA sources. The VLF1, CDR1, CDR2, VLR1 and CDR3 sequences were validated by BLASTp, and values corresponding to IgY V L and V H anti-Ba or anti-Cdt venoms were identified, registered [ Gallus gallus IgY Fv Light chain (GU815099)/ Gallus gallus IgY Fv Heavy chain (GU815098)] and used for molecular modeling of IgY scFv anti-Ba. The resulting CDR1, CDR2 and CDR3 sequences were combined to construct the three - dimensional structure of the Ab paratope. |
