A novel quantitative volumetric spreading index definition and assessment of astrocyte spreading in vitro.

Autor: Tiryaki VM; Department of Computer Engineering, School of Engineering and Architecture, Siirt University, Siirt 56100, Turkey., Ayres VM; Department of Electrical and Computer Engineering, Michigan State University, East Lansing, Michigan 48824., Ahmed I; Department of Biomedical Engineering, Rutgers, The State University of New Jersey, Piscataway, New Jersey 08854., Shreiber DI; Department of Biomedical Engineering, Rutgers, The State University of New Jersey, Piscataway, New Jersey 08854.
Jazyk: angličtina
Zdroj: Cytometry. Part A : the journal of the International Society for Analytical Cytology [Cytometry A] 2017 Aug; Vol. 91 (8), pp. 794-799. Date of Electronic Publication: 2017 Jul 26.
DOI: 10.1002/cyto.a.23183
Abstrakt: A novel quantitative volumetric spreading index (VSI) is defined that depends on the total distance between object voxels and the contact surface plane in three-dimensional (3D) space. The VSI, which ranges from 0 to 1, is rotationally invariant around the z-axis. VSI can be used to quantify the degree of individual cell spreading, which is important for analysis of cell interactions with their environment. The VSIs of astrocytes cultured on a nanofibrillar surface and three different comparative planar surfaces have been calculated from confocal laser scanning microscope z-series images, and the effects of both culture surface and immunoreactivity on the degree of cell spreading were investigated. VSI calculations indicated a statistical correlation between increased reactivity, based on immunolabeling for glial fibrillary acidic protein, and decreased cell spreading. Further results provided a quantitative measure for the increased spreading of quiescent-like and reactive-like astrocytes on planar substrates functionalized with poly-l-lysine. © 2017 International Society for Advancement of Cytometry.
(© 2017 International Society for Advancement of Cytometry.)
Databáze: MEDLINE
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