| Autor: |
Ghiacci G; Dipartimento di Medicina e Chirurgia, Centro Universitario di Odontoiatria, Università degli Studi di Parma, Via Gramsci 14, 43126, Parma. Italy., Graiani G, Cacchioli A, Galli C, Lumetti S, Ravanetti F, Elviri L, Manfredi E, Macaluso GM, Sala R |
| Jazyk: |
angličtina |
| Zdroj: |
Biomedical materials (Bristol, England) [Biomed Mater] 2017 Jul 26; Vol. 12 (4), pp. 045016. Date of Electronic Publication: 2017 Jul 26. |
| DOI: |
10.1088/1748-605X/aa71bc |
| Abstrakt: |
Androgen hormones play a significant role in regulating bone morphogenesis and in maintaining bone homeostasis throughout life. This study aimed to investigate the local effects of the non-aromatizable androgen stanozolol (ST) on bone regeneration in rats. Bilateral critical-size defects were created in the parietal bone of 26 male Wistar rats: the defect on one side was filled with a deproteinized bovine bone scaffold (DBB) soaked in ST solution (test) and the contralateral with DBB alone (control). Samples were collected at one month and three months. Histomorphometry revealed a significantly higher new bone formation (NB) (24.41% ± 4.14% versus 15.01% ± 2.43%, p < 0.05) and mineral apposition rate (MAR) (9.20 μm/day ± 0.37 versus 6.50 μm/day ± 1.09, p < 0.05) in the test versus control group at one month. Accordingly, real time-polymerase chain reaction revealed a consistently higher Runx2 expression in test samples (fold change test/control: 4.50 ± 1.17, p ≤ 0.05). No morphometrical differences between groups were detected at three months (p > 0.05). However, test samples were characterized by an increase in blood capillary density from one month (11.43 n mm -2 ± 2.01) to three months (28.26 n mm -2 ± 5.62), providing evidence of a vital remodeling tissue. Control samples presented a decrease of anti-Osterix (SP7)/anti-osteocalcin (BGLAP) (3.9 n mm -2 ± 0.32 versus 1.01 n mm -2 ± 0.20) and alkaline phosphatase (ALP) (12.14 n mm -2 ± 6.29 versus 6.29 n mm -2 ± 2.73) immunohistochemical-positive elements, which was suggestive of a stabilized healing phase. Based on these observations, local ST administration boosted bone regeneration in rat calvarial critical-size defects at one month. This study showed the potential of local steroid delivery in bone regeneration. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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