Evaluation of intracellular anion superoxide level, heat shock protein A2 and protamine positive spermatozoa percentages in teratoasthenozoospermia.

Autor: Sabeti P; Department of Anatomy, School of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran., Amidi F; Department Of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran., Kalantar SM; Department of Anatomy, School of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran., Sedighi Gilani MA; Department of Urology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran., Pourmasumi S; Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran., Najafi A; Department Of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran., Talebi AR; Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Jazyk: angličtina
Zdroj: International journal of reproductive biomedicine [Int J Reprod Biomed] 2017 May; Vol. 15 (5), pp. 279-286.
Abstrakt: Background: Teratoasthenozoospermia (TA) is a severe form of male infertility with no clear etiology.
Objective: To compare the level of intracellular anion superoxide (O 2 -), heat shock protein A2 (HSPA2) and protamine deficiencies in ejaculated spermatozoa between teratoasthenozoospermic and normozoospermic men.
Materials and Methods: In this case- control study, semen samples of 20 infertile men, with TA (with normal morphology lower than 4%_ and total motility lower than 40% ) as the case group and 20 normozoospermic fertile men as the control group were evaluated for intracellular O 2 - and HSPA2 by flow cytometry and protamine deficiency by Chromomycin A3 (CMA3) test.
Results: The rate of CMA3+ spermatozoa in the case group was higher than controls (p=0.001). The percentages of HSPA2+ spermatozoa in the cases were significantly lower than controls (p=0.001). Also, intracellular O 2 - levels in the case group were significantly higher than controls (p=0.001) and had positive correlations with sperm apoptosis (r=0.79, p=0.01) and CMA3 positive sperm (r=0.76, p=0.01), but negative correlations with normal morphology (r=-0.81, p=0.01) and motility (r=-0.81, p=0.01). There was no significant correlation between intracellular O 2 - and HSPA2 in the case group (r=0.041, p=0.79).
Conclusion: We suggest that the increase in intracellular O 2 - , decrease in spermatozoa HSPA2 + , and high percentages of spermatozoa with immature chromatin might be considered as etiologies of infertility in TA patients.
Competing Interests: There is no conflict of interest in this article.
Databáze: MEDLINE
Externí odkaz: