Imaging regiospecific lipid turnover in mouse brain with desorption electrospray ionization mass spectrometry.

Autor:	Carson RH; Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 86402., Lewis CR; Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 86402., Erickson MN; Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 86402., Zagieboylo AP; Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 86402., Naylor BC; Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 86402., Li KW; Independent Researcher Brigham Young University, Provo, UT 86402., Farnsworth PB; Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 86402. Electronic address: paul_farnsworth@byu.edu., Price JC; Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 86402. Electronic address: jcprice@chem.byu.edu.
Jazyk:	angličtina
Zdroj:	Journal of lipid research [J Lipid Res] 2017 Sep; Vol. 58 (9), pp. 1884-1892. Date of Electronic Publication: 2017 Jul 25.
DOI:	10.1194/jlr.M078170
Abstrakt:	Compartmentalization of metabolism into specific regions of the cell, tissue, and organ is critical to life for all organisms. Mass spectrometric imaging techniques have been valuable in identifying and quantifying concentrations of metabolites in specific locations of cells and tissues, but a true understanding of metabolism requires measurement of metabolite flux on a spatially resolved basis. Here, we utilize desorption ESI-MS (DESI-MS) to measure lipid turnover in the brains of mice. We show that anatomically distinct regions of the brain have distinct lipid turnover rates. These turnover measurements, in conjunction with relative concentration, will enable calculation of regiospecific synthesis rates for individual lipid species in vivo. Monitoring spatially dependent changes in metabolism has the potential to significantly facilitate research in many areas, such as brain development, cancer, and neurodegeneration. (Copyright © 2017 by the American Society for Biochemistry and Molecular Biology, Inc.)
Databáze:	MEDLINE
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