Green fluorescent protein (GFP): is seeing believing and is that enough?

Autor: Shorter SA; a IDS Laboratory, Department of Life and Sports Science, Faculty of Engineering and Science , University of Greenwich , Chatham , Kent , UK., Pettit MW; a IDS Laboratory, Department of Life and Sports Science, Faculty of Engineering and Science , University of Greenwich , Chatham , Kent , UK., Dyer PDR; a IDS Laboratory, Department of Life and Sports Science, Faculty of Engineering and Science , University of Greenwich , Chatham , Kent , UK., Coakley Youngs E; a IDS Laboratory, Department of Life and Sports Science, Faculty of Engineering and Science , University of Greenwich , Chatham , Kent , UK., Gorringe-Pattrick MAM; a IDS Laboratory, Department of Life and Sports Science, Faculty of Engineering and Science , University of Greenwich , Chatham , Kent , UK., El-Daher S; a IDS Laboratory, Department of Life and Sports Science, Faculty of Engineering and Science , University of Greenwich , Chatham , Kent , UK., Richardson S; a IDS Laboratory, Department of Life and Sports Science, Faculty of Engineering and Science , University of Greenwich , Chatham , Kent , UK.
Jazyk: angličtina
Zdroj: Journal of drug targeting [J Drug Target] 2017 Nov - Dec; Vol. 25 (9-10), pp. 809-817. Date of Electronic Publication: 2017 Aug 09.
DOI: 10.1080/1061186X.2017.1358725
Abstrakt: Intracellular compartmentalisation is a significant barrier to the successful nucleocytosolic delivery of biologics. The endocytic system has been shown to be responsible for compartmentalisation, providing an entry point, and trigger(s) for the activation of drug delivery systems. Consequently, many of the technologies used to understand endocytosis have found utility within the field of drug delivery. The use of fluorescent proteins as markers denoting compartmentalisation within the endocytic system has become commonplace. Several of the limitations associated with the use of green fluorescent protein (GFP) within the context of drug delivery have been explored here by asking a series of related questions: (1) Are molecules that regulate fusion to a specific compartment (i.e. Rab- or SNARE-GFP fusions) a good choice of marker for that compartment? (2) How reliable was GFP-marker overexpression when used to define a given endocytic compartment? (3) Can glutathione-s-transferase (GST) fused in frame with GFP (GST-GFP) act as a fluid phase endocytic probe? (4) Was GFP fluorescence a robust indicator of (GFP) protein integrity? This study concluded that there are many appropriate and useful applications for GFP; however, thought and an understanding of the biological and physicochemical character of these markers are required for the generation of meaningful data.
Databáze: MEDLINE
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