Role of 5α-dihydrotestosterone in testicular development of gilthead seabream following finasteride administration.

Autor: García-García M; Sección de Microscopía, Servicio de Apoyo a la Investigación Regional, Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, Murcia 30100, Spain., Sánchez-Hernández M; Centro Oceanográfico de Murcia, Instituto Español de Oceanografía, Puerto de Mazarrón, 30860, Spain., García-Hernández MP; Department of Cell Biology and Histology, Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, Murcia, 30100, Spain., García-Ayala A; Department of Cell Biology and Histology, Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, Murcia, 30100, Spain., Chaves-Pozo E; Centro Oceanográfico de Murcia, Instituto Español de Oceanografía, Puerto de Mazarrón, 30860, Spain. Electronic address: elena.chaves@mu.ieo.es.
Jazyk: angličtina
Zdroj: The Journal of steroid biochemistry and molecular biology [J Steroid Biochem Mol Biol] 2017 Nov; Vol. 174, pp. 48-55. Date of Electronic Publication: 2017 Jul 21.
DOI: 10.1016/j.jsbmb.2017.07.024
Abstrakt: In teleosts, spermatogenesis is regulated by pituitary gonadotropins and sex steroids. 5α-dihydrotestosterone (DHT), derived from testosterone (T) through the action of 5α-reductase, has recently been suggested to play a physiologically important role in some fish species. In this study, gilthead seabream, Sparus aurata L., males received an implant of 1μgT/g body mass (bm) or vehicle alone and, 7days later, 1mg finasteride (FIN, an inhibitor of 5α-reductase)/kg bm or vehicle. Serum levels of T, 11-ketotestosterone (11KT), DHT and 17β-estradiol (E 2 ), and the mRNA levels of the main enzymes involved in their synthesis, were analysed. T promoted a transient increase in the serum levels of T, 11KT and E 2 but a decrease in those of DHT at day 15 following T injection, in accordance with the up-regulation of mRNA levels of the enzymes involved in T transformation to 11KT (coding genes: cyp11b1 and hsd11b) and the down-regulation of mRNA levels of the enzyme responsible for T transformation to DHT (coding gene: srd5a). Interestingly, a similar effect was observed when FIN was injected. However, when fish were injected with T and FIN successively (T+FIN), control levels were not recovered at the end of the experimental period (28days). DHT seems to regulate E 2 serum levels via the down-regulation of mRNA levels of aromatase (coding gene: cyp19a1a), which is needed for the transformation of T into E 2 . The testis histology, together with the proliferative rates recorded upon T, FIN or T+FIN treatment, suggests that DHT is involved in the onset of the meiotic phase of spermatogenesis.
(Copyright © 2017 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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