Phenolic compounds of Triplaris gardneriana can protect cells against oxidative stress and restore oxidative balance.

Autor: de Almeida TS; Department of Biochemistry and Molecular Biology, Federal University of Ceará, 60020-181, Fortaleza, CE, Brazil., Neto JJL; Department of Biochemistry and Molecular Biology, Federal University of Ceará, 60020-181, Fortaleza, CE, Brazil., de Sousa NM; Department of Biochemistry and Molecular Biology, Federal University of Ceará, 60020-181, Fortaleza, CE, Brazil., Pessoa IP; Department of Biochemistry and Molecular Biology, Federal University of Ceará, 60020-181, Fortaleza, CE, Brazil., Vieira LR; Department of Biochemistry and Molecular Biology, Federal University of Ceará, 60020-181, Fortaleza, CE, Brazil., de Medeiros JL; Department of Biochemistry and Molecular Biology, Federal University of Ceará, 60020-181, Fortaleza, CE, Brazil., Boligon AA; Pharmaceutical Sciences, Federal University of Santa Maria, 97105-900, Santa Maria, RS, Brazil., Hamers ARM; Rikilt Institute of Food Safety, Wageningen University and Research Centre, P.O. Box 230, 6700 AE Wageningen, The Netherlands., Farias DF; Department of Molecular Biology, Federal University of Paraíba, 58051-900, João Pessoa, PB, Brazil., Peijnenburg A; Rikilt Institute of Food Safety, Wageningen University and Research Centre, P.O. Box 230, 6700 AE Wageningen, The Netherlands., Carvalho AFU; Department of Biochemistry and Molecular Biology, Federal University of Ceará, 60020-181, Fortaleza, CE, Brazil. Electronic address: aurano@ufc.br.
Jazyk: angličtina
Zdroj: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie [Biomed Pharmacother] 2017 Sep; Vol. 93, pp. 1261-1268. Date of Electronic Publication: 2017 Jul 20.
DOI: 10.1016/j.biopha.2017.07.050
Abstrakt: This work aimed to add value to an underexploited plant species from Brazil, Triplaris gardneriana. To that, the phenolic compounds profile of its seed ethanolic extract and fractions was examined by HPLC and the antioxidant capacity assessed using chemical assays as well as in vitro cell imaging. Twelve compounds were quantified and classified as either phenolic acids or flavonoids. The fractionation process did not generate fractions with different compositions except for chloroformic fraction, which showed only 6 out of 12 standard compounds used. DPPH assay revealed samples with a concentration-dependent radical scavenging activity, being methanolic fraction the one with the largest activity (SC 50 11.45±0.02μg/mL). Lipid peroxidation assessment, in the presence and absence of stress inducer, showed that particularly the ethanol extract (IC 50 26.75±0.08μg/mL) and the ethyl acetate fraction (IC 50 6.14±0.03μg/mL) could inhibit lipid peroxidation. The ethyl acetate fraction performed best in chelating iron (48% complexation at 1000μg/mL). Cell imaging experiments showed that the ethanolic extract could protect cells against oxidative stress as well as restore the oxidative balance upon stress induction. In conclusion, T. gardneriana seeds showed a promising phenolic compounds profile and antioxidant activity that may be further exploited.
(Copyright © 2017 Elsevier Masson SAS. All rights reserved.)
Databáze: MEDLINE
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