Changes in optical properties of electroporated cells as revealed by digital holographic microscopy.

Autor: Calin VL; Biophysics and Cellular Biotechnology Dept., Faculty of Medicine, Carol Davila University of Medicine and Pharmacy, 8 Eroii Sanitari Blvd., Bucharest 050474, Romania., Mihailescu M; Physics Dept., Faculty of Applied Sciences, Politehnica University of Bucharest, 313 Spl. Independentei, Bucharest 060042, Romania., Mihale N; Physics Dept., Faculty of Applied Sciences, Politehnica University of Bucharest, 313 Spl. Independentei, Bucharest 060042, Romania., Baluta AV; Applied Electronics and Informatics Engineering Dept., Faculty of Electronics, Telecommunications and Information Technology, Politehnica University of Bucharest, 313 Spl. Independentei, Bucharest 060042, Romania., Kovacs E; Biophysics and Cellular Biotechnology Dept., Faculty of Medicine, Carol Davila University of Medicine and Pharmacy, 8 Eroii Sanitari Blvd., Bucharest 050474, Romania., Savopol T; Biophysics and Cellular Biotechnology Dept., Faculty of Medicine, Carol Davila University of Medicine and Pharmacy, 8 Eroii Sanitari Blvd., Bucharest 050474, Romania., Moisescu MG; Biophysics and Cellular Biotechnology Dept., Faculty of Medicine, Carol Davila University of Medicine and Pharmacy, 8 Eroii Sanitari Blvd., Bucharest 050474, Romania.
Jazyk: angličtina
Zdroj: Biomedical optics express [Biomed Opt Express] 2017 Mar 16; Vol. 8 (4), pp. 2222-2234. Date of Electronic Publication: 2017 Mar 16 (Print Publication: 2017).
DOI: 10.1364/BOE.8.002222
Abstrakt: Changes in optical and shape-related characteristics of B16F10 cells after electroporation were investigated using digital holographic microscopy (DHM). Bipolar rectangular pulses specific for electrochemotherapy were used. Electroporation was performed in an "off-axis" DHM set-up without using exogenous markers. Two types of cell parameters were monitored seconds and minutes after pulse train application: parameters addressing a specifically defined area of the cell (refractive index and cell height) and global cell parameters (projected area, optical phase shift profile and dry mass). The biphasic behavior of cellular parameters was explained by water and mannitol dynamics through the electropermeabilized cell membrane.
Databáze: MEDLINE
Externí odkaz: