Pneumonia due to Talaromyces marneffei in a Dog from Southern Brazil with Concomitant Canine Distemper Virus Infection.

Autor: Headley SA; Laboratory of Animal Pathology, Universidade Estadual de Londrina, Paraná, Brazil. Electronic address: selwyn.headley@uel.br., Pretto-Giordano LG; Laboratory of Mycology, Universidade Estadual de Londrina, Paraná, Brazil., Lima SC; Laboratory of Genetics and Bacterial Taxonomy, Department of General Biology, Universidade Estadual de Londrina, Paraná, Brazil., Suhett WG; Small Animal Internal Medicine, Department of Veterinary Clinics, Universidade Estadual de Londrina, Paraná, Brazil., Pereira AHT; Laboratory of Animal Pathology, Universidade Estadual de Londrina, Paraná, Brazil., Freitas LA; Laboratory of Molecular Biology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Paraná, Brazil., Suphoronski SA; Laboratory of Mycology, Universidade Estadual de Londrina, Paraná, Brazil., Oliveira TES; Laboratory of Animal Pathology, Universidade Estadual de Londrina, Paraná, Brazil., Alfieri AF; Laboratory of Molecular Biology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Paraná, Brazil., Pereira EC; Laboratory of Mycology, Universidade Estadual de Londrina, Paraná, Brazil., Vilas-Boas LA; Laboratory of Genetics and Bacterial Taxonomy, Department of General Biology, Universidade Estadual de Londrina, Paraná, Brazil., Alfieri AA; Multi-User Animal Health Laboratory, Molecular Biology Unit, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Paraná, Brazil.
Jazyk: angličtina
Zdroj: Journal of comparative pathology [J Comp Pathol] 2017 Jul; Vol. 157 (1), pp. 61-66. Date of Electronic Publication: 2017 Jul 05.
DOI: 10.1016/j.jcpa.2017.06.001
Abstrakt: The pathological and molecular findings associated with Talaromyces marneffei-induced pneumonia with concomitant infection by canine distemper virus (CDV) are described in a dog. The principal pathological alteration occurred in the lungs. Histopathology confirmed multifocal granulomatous pneumonia associated with numerous intralesional and intracellular septate fission cells consistent with T. marneffei. A molecular assay designed to amplify a partial fragment of the 18S rRNA gene of T. marneffei provided positive results from two fungal cultures derived from the lung. Sequencing and phylogenetic analyses confirmed the results of polymerase chain reaction (PCR). Furthermore, antigens of the CDV N protein were identified within the bronchial epithelium by immunohistochemistry and a PCR assay amplified the CDV N gene from hepatic and pulmonary fragments. Collectively, the pathological and molecular techniques confirmed a diagnosis of T. marneffei-induced pneumonia with concomitant infection by CDV. These findings represent the first description of pulmonary penicilliosis in the dog and extend the geographical niche of this emerging infectious pathogen. In this case, infection by CDV may have induced immunosuppression, which facilitated the development of pulmonary penicilliosis.
(Copyright © 2017 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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