Androgen-responsive non-coding small RNAs extend the potential of HCG stimulation to act as a bioassay of androgen sufficiency.
| Autor: | Rodie ME; Developmental Endocrinology Research Group, University of Glasgow, Glasgow, UK., Mudaliar MAV; Glasgow Polyomics.; Glasgow Molecular Pathology Node., Herzyk P; Glasgow Polyomics.; Institute of Molecular Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, UK., McMillan M; Developmental Endocrinology Research Group, University of Glasgow, Glasgow, UK., Boroujerdi M; Developmental Endocrinology Research Group, University of Glasgow, Glasgow, UK., Chudleigh S; Department of Molecular Haematology., Tobias ES; Developmental Endocrinology Research Group, University of Glasgow, Glasgow, UK.; Glasgow Molecular Pathology Node.; Academic Medical Genetics and Pathology, Laboratory Medicine Building, Queen Elizabeth University Hospital, University of Glasgow, Glasgow, UK., Ahmed SF; Developmental Endocrinology Research Group, University of Glasgow, Glasgow, UK. |
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| Jazyk: | angličtina |
| Zdroj: | European journal of endocrinology [Eur J Endocrinol] 2017 Oct; Vol. 177 (4), pp. 339-346. Date of Electronic Publication: 2017 Jul 21. |
| DOI: | 10.1530/EJE-17-0404 |
| Abstrakt: | Background: It is unclear whether a short-term change in circulating androgens is associated with changes in the transcriptome of the peripheral blood mononuclear cells (PBMC). Aims and Methods: To explore the effect of hCG stimulation on the PBMC transcriptome, 12 boys with a median age (range) of 0.7 years (0.3, 11.2) who received intramuscular hCG 1500u on 3 consecutive days as part of their investigations underwent transcriptomic array analysis on RNA extracted from peripheral blood mononuclear cells before and after hCG stimulation. Results: Median pre- and post-hCG testosterone for the overall group was 0.7 nmol/L (<0.5, 6) and 7.9 nmol/L (<0.5, 31.5), respectively. Of the 12 boys, 3 (25%) did not respond to hCG stimulation with a pre and post median serum testosterone of <0.5 nmol/L and <0.5 nmol/L, respectively. When corrected for gene expression changes in the non-responders to exclude hCG effects, all 9 of the hCG responders consistently demonstrated a 20% or greater increase in the expression of piR-37153 and piR-39248 , non-coding PIWI-interacting RNAs (piRNAs). In addition, of the 9 responders, 8, 6 and 4 demonstrated a 30, 40 and 50% rise, respectively, in a total of 2 further piRNAs. In addition, 3 of the responders showed a 50% or greater rise in the expression of another small RNA, SNORD5 . On comparing fold-change in serum testosterone with fold-change in the above transcripts, a positive correlation was detected for SNORD5 ( P = 0.01). Conclusions: The identification of a dynamic and androgen-responsive PBMC transcriptome extends the potential value of the hCG test for the assessment of androgen sufficiency. (© 2017 The authors.) |
| Databáze: | MEDLINE |
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