Glycerol-3-phosphate acyltransferase 2 is essential for normal spermatogenesis.
Autor: | Garcia-Fabiani MB; Instituto de Investigaciones Bioquímicas de La Plata 'Rodolfo R. Brenner', Consejo Nacional de Investigaciones Científicas y Técnicas, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, La Plata 1900, Argentina., Montanaro MA; Instituto de Investigaciones Bioquímicas de La Plata 'Rodolfo R. Brenner', Consejo Nacional de Investigaciones Científicas y Técnicas, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, La Plata 1900, Argentina., Stringa P; Laboratorio de Trasplante de Órganos, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, La Plata 1900, Argentina., Lacunza E; Centro de Investigaciones Inmunológicas Básicas y Aplicadas, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, La Plata 1900, Argentina., Cattaneo ER; Instituto de Investigaciones Bioquímicas de La Plata 'Rodolfo R. Brenner', Consejo Nacional de Investigaciones Científicas y Técnicas, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, La Plata 1900, Argentina., Santana M; Instituto de Investigaciones Bioquímicas de La Plata 'Rodolfo R. Brenner', Consejo Nacional de Investigaciones Científicas y Técnicas, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, La Plata 1900, Argentina., Pellon-Maison M; Instituto de Investigaciones Bioquímicas de La Plata 'Rodolfo R. Brenner', Consejo Nacional de Investigaciones Científicas y Técnicas, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, La Plata 1900, Argentina., Gonzalez-Baro MR; Instituto de Investigaciones Bioquímicas de La Plata 'Rodolfo R. Brenner', Consejo Nacional de Investigaciones Científicas y Técnicas, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, La Plata 1900, Argentina mgbaro@med.unlp.edu.ar. |
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Jazyk: | angličtina |
Zdroj: | The Biochemical journal [Biochem J] 2017 Aug 30; Vol. 474 (18), pp. 3093-3107. Date of Electronic Publication: 2017 Aug 30. |
DOI: | 10.1042/BCJ20161018 |
Abstrakt: | Glycerol-3-phosphate acyltransferases (GPATs) catalyze the first and rate-limiting step in the de novo glycerolipid synthesis. The GPAT2 isoform differs from the other isoforms because its expression is restricted to male germ cells and cancer cells. It has been recently reported that GPAT2 expression in mouse testis fluctuates during sexual maturation and that it is regulated by epigenetic mechanisms in combination with vitamin A derivatives. Despite progress made in this field, information about GPAT2 role in the developing male germ cells remains unclear. The aim of the present study was to confirm the hypothesis that GPAT2 is required for the normal physiology of testes and male germ cell maturation. The gene was silenced in vivo by inoculating lentiviral particles carrying the sequence of a short-hairpin RNA targeting Gpat2 mRNA into mouse testis. Histological and gene expression analysis showed impaired spermatogenesis and arrest at the pachytene stage. Defects in reproductive fitness were also observed, and the analysis of apoptosis-related gene expression demonstrated the activation of apoptosis in Gpat2 -silenced germ cells. These findings indicate that GPAT2 protein is necessary for the normal development of male gonocytes, and that its absence triggers apoptotic mechanisms, thereby decreasing the number of dividing germ cells. (© 2017 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society.) |
Databáze: | MEDLINE |
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