Antibacterial activity of extract and fractions from branches of Protium spruceanum and cytotoxicity on fibroblasts.

Autor: Amparo TR; a Departamento de Farmácia , Universidade Federal de Ouro Preto , Ouro Preto , Brazil., Rodrigues IV; b Departamento de Farmácia , Universidade Federal de Juiz de Fora , Governador Valadares , Brazil., Seibert JB; a Departamento de Farmácia , Universidade Federal de Ouro Preto , Ouro Preto , Brazil., Souza RHZ; a Departamento de Farmácia , Universidade Federal de Ouro Preto , Ouro Preto , Brazil., Oliveira AR; a Departamento de Farmácia , Universidade Federal de Ouro Preto , Ouro Preto , Brazil., Cabral VAR; c Departamento de Ciências Florestais , Universidade Federal de Lavras , Lavras , Brazil., Vieira PMA; d Departamento de Ciências Biológicas , Universidade Federal de Ouro Preto , Ouro Preto , Brazil., Brandão GC; a Departamento de Farmácia , Universidade Federal de Ouro Preto , Ouro Preto , Brazil., Okuma AA; e Departamento de Química , Centro Federal de Educação Tecnológica de Minas Gerais , Belo Horizonte , Brazil., Filho SAV; a Departamento de Farmácia , Universidade Federal de Ouro Preto , Ouro Preto , Brazil., Teixeira LFM; a Departamento de Farmácia , Universidade Federal de Ouro Preto , Ouro Preto , Brazil., Souza GHB; a Departamento de Farmácia , Universidade Federal de Ouro Preto , Ouro Preto , Brazil.
Jazyk: angličtina
Zdroj: Natural product research [Nat Prod Res] 2018 Aug; Vol. 32 (16), pp. 1951-1954. Date of Electronic Publication: 2017 Jul 20.
DOI: 10.1080/14786419.2017.1354182
Abstrakt: The crude ethanol extract (CEE) and fractions from branches of Protium spruceanum were subjected to antibacterial and cytotoxicity assays. Compounds of the most active fraction were identified by GC-MS and LC-MS. CEE was active against 19 bacteria and the ethyl acetate fraction (EAF) showed the lowest minimum bactericidal concentration (MBC 0.3-80.0 mg/mL). Through time-kill assay was observed that EAF induced rapid bactericidal effect against Staphylococcus saprophyticus. The cytotoxicity tests against L929 fibroblasts showed great potential of EAF on the treatment of infections caused by five bacteria (MBC < IC 50 ). The results provide in vitro scientific support to the possible application of branches of P. spruceanum as antimicrobial agent that may contribute for treatment of infections.
Databáze: MEDLINE