Characteristics of Mel Ibr Melanoma Line Subclone after Treatment with Chicken Embryo Extract.

Autor: Suraeva NM; N. N. Blokhin Russian Cancer Research Center, Ministry of Health of the Russian Federation, Moscow, Russia. nsuraeva@yandex.ru., Morozova LF; N. N. Blokhin Russian Cancer Research Center, Ministry of Health of the Russian Federation, Moscow, Russia., Ryabaya OO; N. N. Blokhin Russian Cancer Research Center, Ministry of Health of the Russian Federation, Moscow, Russia., Khochenkova YA; N. N. Blokhin Russian Cancer Research Center, Ministry of Health of the Russian Federation, Moscow, Russia., Samoilov AV; All-Russian Research Institute of Preservation Technology, Moscow Region, Russia., Burova OS; N. N. Blokhin Russian Cancer Research Center, Ministry of Health of the Russian Federation, Moscow, Russia., Golubtsova NV; N. N. Blokhin Russian Cancer Research Center, Ministry of Health of the Russian Federation, Moscow, Russia., Barmashov AE; N. N. Blokhin Russian Cancer Research Center, Ministry of Health of the Russian Federation, Moscow, Russia., Baryshnikova MA; N. N. Blokhin Russian Cancer Research Center, Ministry of Health of the Russian Federation, Moscow, Russia.
Jazyk: angličtina
Zdroj: Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2017 Jun; Vol. 163 (2), pp. 255-259. Date of Electronic Publication: 2017 Jul 18.
DOI: 10.1007/s10517-017-3778-y
Abstrakt: We continue analysis of the phenotype of human melanoma cell Mel Ibr subclone obtained previously by treatment of the parental cell line by chicken embryo extract. The present study is focused on detection of markers of epithelial-mesenchymal transition that determine enhanced metastatic and invasive potential of malignant tumors of various locations. Analysis of the expression of E-cadherin and vimentin genes in the subclone and parental cells detected activation of epithelial-mesenchymal transition in the subclone. Immunological markers CD90, CD271, and CD95 were present in the parental population, but were not detected on the subclone cells. In contrast to the parental line, cells of the analyzed subclone retain viability in serum-free medium and formed vessel-like structures characteristic of vasculogenic mimicry.
Databáze: MEDLINE
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