Regenerative Potential of Spermatogonial Stem Cells, Endothelial Progenitor Cells, and Epithelial Progenitor Cells of C57Bl/6 Male Mice with Metabolic Disorders.

Autor: Skurikhin EG; E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk, Russia., Pakhomova AV; E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk, Russia. angelinapakhomova2011@gmail.com., Pershina OV; E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk, Russia., Ermolaeva LA; E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk, Russia., Krupin VA; E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk, Russia., Ermakova NN; E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk, Russia., Pan ES; E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk, Russia., Kudryashova AI; E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk, Russia., Rybalkina OY; E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk, Russia., Pavlovskaya TB; E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk, Russia., Litvyakov NV; Tomsk National Research Medical Center, Russian Academy of Sciences, Tomsk, Russia., Goldberg VE; Tomsk National Research Medical Center, Russian Academy of Sciences, Tomsk, Russia., Dygai AM; E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk, Russia.
Jazyk: angličtina
Zdroj: Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2017 Jun; Vol. 163 (2), pp. 239-244. Date of Electronic Publication: 2017 Jul 18.
DOI: 10.1007/s10517-017-3775-1
Abstrakt: The properties of spermatogonial stem cells, endothelial progenitor cells, and the epithelial progenitors of C57Bl/6 mice under conditions of metabolic disorders were studied using the model of busulfan-induced suppression of spermatogenesis and in vitro culture technique. Spermatogonial stem cells CD117-CD90 + and epithelial progenitors CD45-CD31-Sca-1 + CD49f + derived from the testes of mice with metabolic disturbances demonstrated 17- and 28-fold increase in the respective cell mass and generated cell colonies in vitro. In contrast, spermatogonial stem cells with immune phenotype CD51-CD24 + CD52 + had reduced selfrenewal capacity. Spermatogonial stem cells CD117-CD90 + and CD117 + CD90 + as well as endothelial progenitors CD45-CD31 + derived from the testes of donor mice with metabolic disorders demonstrated high transplantation capacity in C57Bl/6 mouse testes damaged by cytostatic busulfan.
Databáze: MEDLINE
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