Immunophenotypic aberrancies in acute lymphoblastic leukemia from 282 Iraqi patients.

Autor: Jalal SD; Department of Pathology, College of Medicine, University of Sulaimani, Sulaimani, Iraq., Al-Allawi NAS; Department of Pathology, College of Medicine, University of Duhok, Duhok, Iraq., Al Doski AAS; Department of Pathology, College of Medicine, University of Duhok, Duhok, Iraq.
Jazyk: angličtina
Zdroj: International journal of laboratory hematology [Int J Lab Hematol] 2017 Dec; Vol. 39 (6), pp. 625-632. Date of Electronic Publication: 2017 Jul 18.
DOI: 10.1111/ijlh.12716
Abstrakt: Introduction: The identification of aberrancies in leukemia-associated immunophenotype (LAIP) of acute lymphoblastic leukemia (ALL) is quite important in the assessment of minimal residual disease (MRD). This study, the first from Iraq, aimed to assess the frequency and patterns of LAIP among Iraqi patients with ALL, to establish future strategies for evaluating MRD.
Methods: A total of 282 newly diagnosed Iraqi ALL cases were analyzed with six-parameter flow cytometry using a panel of 29 monoclonal antibodies.
Results: Immunological subtyping revealed that 85.5% of cases were B-ALL and the remainder T-ALL. LAIP was detected in 97.1% of B-ALL, and in 26.8% of T-ALL. The asynchronous maturation-associated antigen patterns in B-ALL were CD10 strong+ /TdT dim+ , CD38 dim+ /CD34 + , CD10 dim+ /CD34 + , CD10 strong /CD20 strong+ , CD20 strong+ /CD34 +, and CD45 dim+ /CD20 strong+ in 84.6%, while the cross-lineage myeloid expression was seen in 81.3% and aberrant T-cell antigen expression in 6.2%. For T-ALL, asynchronous maturation-associated antigen patterns included the following: CD1a + /CD5 + /sCD3 + and CD34 + /sCD3 + in 12.2%. Myeloid and B-cell antigen expression were each identified in 7.3% of T-ALL. No significant differences in LAIP were found between children and adults.
Conclusion: The high rates and the patterns of LAIP particularly in Iraqi B-ALL patients may allow the development of more cost-effective strategies for MRD monitoring.
(© 2017 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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