Reversible meiotic arrest of bovine oocytes by EGFR inhibition and follicular hemisections.

Autor: da Rosa PRA; Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University of Santa Maria, Santa Maria, RS, Brazil; Department of Animal Science, McGill University, Sainte-Anne-de-Bellevue, QC, Canada., De Cesaro MP; Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University of Santa Maria, Santa Maria, RS, Brazil., Pereira Dau AM; Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University of Santa Maria, Santa Maria, RS, Brazil., Duggavathi R; Department of Animal Science, McGill University, Sainte-Anne-de-Bellevue, QC, Canada., Bordignon V; Department of Animal Science, McGill University, Sainte-Anne-de-Bellevue, QC, Canada. Electronic address: vilceu.bordignon@mcgill.ca., Gonçalves PBD; Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University of Santa Maria, Santa Maria, RS, Brazil. Electronic address: bayard@ufsm.br.
Jazyk: angličtina
Zdroj: Theriogenology [Theriogenology] 2017 Sep 01; Vol. 99, pp. 53-62. Date of Electronic Publication: 2017 May 23.
DOI: 10.1016/j.theriogenology.2017.05.014
Abstrakt: The objective of this study was to investigate the effects of inhibiting the epidermal growth factor receptor (EGFR) pathway on meiosis blockage and resumption, mRNA expression of genes involved in oocyte maturation and cumulus expansion, and embryo development. Bovine cumulus-oocyte complexes (COCs) were cultured for 15 h in the presence of the EGFR inhibitor (AG1478) and follicular hemisections (FHS). Most of the oocytes (89.3%) remained at the germinal vesicle (GV) stage when cultured in the presence of FHS and 5 μM AG1478. The inhibitory effect was reversible as most oocytes (83.8%) completed meiosis after additional 20 h maturation. Embryo development to the blastocyst stage was similar (P > 0.05) between FHS and 5 μM AG1478 treated (39.3%) and control (41.1%) groups. In cumulus cells, mRNA abundance of early growth response protein 1 (EGR1), tumor necrosis factor alpha-induced protein 6 (TNFAIP6) and hyaluronan synthase 2 (HAS2) genes, and phosphorylated extracellular regulated kinase (p-ERK1/2) protein were lower in COCs treated with AG1478 plus FHS compared with FHS alone (P < 0.05). In granulosa cells of FHS, AG1478 treatment reduced transcript levels of PGR and ADAMTS1 (P < 0.05). The inhibitory effect of AG1478 on meiotic progression was not reverted by treatment with angiotensin II (ANG II) or prostaglandins (PGF 2α or PGE 2 ). This study demonstrates that inhibition of EGFR in the presence of FHS is a reliable approach to promote reversible arrest of bovine oocytes at the GV stage.
(Copyright © 2017. Published by Elsevier Inc.)
Databáze: MEDLINE
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