| Autor: |
Sun X; Department of Chemistry , University of Bath , Bath , BA2 7AY , UK . Email: t.d.james@bath.ac.uk ; Email: s.d.bull@bath.ac.uk., Lacina K; Department of Chemistry , University of Bath , Bath , BA2 7AY , UK . Email: t.d.james@bath.ac.uk ; Email: s.d.bull@bath.ac.uk.; CEITEC , Masaryk University , Kamenice 5 , 62500 , Brno , Czech Republic., Ramsamy EC; Department of Chemistry , University of Bath , Bath , BA2 7AY , UK . Email: t.d.james@bath.ac.uk ; Email: s.d.bull@bath.ac.uk., Flower SE; Department of Chemistry , University of Bath , Bath , BA2 7AY , UK . Email: t.d.james@bath.ac.uk ; Email: s.d.bull@bath.ac.uk., Fossey JS; School of Chemistry , University of Birmingham , Edgbaston , Birmingham , West Midlands , B15 2TT , UK., Qian X; School of Pharmacy , East China University of Science and Technology , Meilong Road 130 , Shanghai 200237 , China., Anslyn EV; Department of Chemistry and Biochemistry , The University of Texas at Austin , Austin , Texas 78712 , USA . Email: anslyn@austin.utexas.edu., Bull SD; Department of Chemistry , University of Bath , Bath , BA2 7AY , UK . Email: t.d.james@bath.ac.uk ; Email: s.d.bull@bath.ac.uk., James TD; Department of Chemistry , University of Bath , Bath , BA2 7AY , UK . Email: t.d.james@bath.ac.uk ; Email: s.d.bull@bath.ac.uk. |
| Abstrakt: |
Using the self-assembly of aromatic boronic acids with Alizarin Red S (ARS), we developed a new chemosensor for the selective detection of peroxynitrite. Phenylboronic acid (PBA), benzoboroxole (BBA) and 2-( N , N -dimethylaminomethyl)phenylboronic acid (NBA) were employed to bind with ARS to form the complex probes. In particular, the ARS-NBA system with a high binding affinity can preferably react with peroxynitrite over hydrogen peroxide and other ROS/RNS due to the protection of the boron via the solvent-insertion B-N interaction. Our simple system produces a visible colorimetric change and on-off fluorescence response towards peroxynitrite. By coupling a chemical reaction that leads to an indicator displacement, we have developed a new sensing strategy, referred to herein as RIA (Reaction-based Indicator displacement Assay). |
