Octamer formation in lysozyme solutions at the initial crystallization stage detected by small-angle neutron scattering.

Autor: Boikova AS; Shubnikov Institute of Crystallography, Federal Scientific Research Centre `Crystallography and Photonics', Russian Academy of Sciences, Leninskii pr. 59, Moscow 119333, Russian Federation., Dyakova YA; Shubnikov Institute of Crystallography, Federal Scientific Research Centre `Crystallography and Photonics', Russian Academy of Sciences, Leninskii pr. 59, Moscow 119333, Russian Federation., Ilina KB; Shubnikov Institute of Crystallography, Federal Scientific Research Centre `Crystallography and Photonics', Russian Academy of Sciences, Leninskii pr. 59, Moscow 119333, Russian Federation., Konarev PV; Shubnikov Institute of Crystallography, Federal Scientific Research Centre `Crystallography and Photonics', Russian Academy of Sciences, Leninskii pr. 59, Moscow 119333, Russian Federation., Kryukova AE; Shubnikov Institute of Crystallography, Federal Scientific Research Centre `Crystallography and Photonics', Russian Academy of Sciences, Leninskii pr. 59, Moscow 119333, Russian Federation., Kuklin AI; The Joint Institute for Nuclear Research, Joliot-Curie str. 6, Dubna 141980, Russian Federation., Marchenkova MA; Shubnikov Institute of Crystallography, Federal Scientific Research Centre `Crystallography and Photonics', Russian Academy of Sciences, Leninskii pr. 59, Moscow 119333, Russian Federation., Nabatov BV; Shubnikov Institute of Crystallography, Federal Scientific Research Centre `Crystallography and Photonics', Russian Academy of Sciences, Leninskii pr. 59, Moscow 119333, Russian Federation., Blagov AE; Shubnikov Institute of Crystallography, Federal Scientific Research Centre `Crystallography and Photonics', Russian Academy of Sciences, Leninskii pr. 59, Moscow 119333, Russian Federation., Pisarevsky YV; Shubnikov Institute of Crystallography, Federal Scientific Research Centre `Crystallography and Photonics', Russian Academy of Sciences, Leninskii pr. 59, Moscow 119333, Russian Federation., Kovalchuk MV; Shubnikov Institute of Crystallography, Federal Scientific Research Centre `Crystallography and Photonics', Russian Academy of Sciences, Leninskii pr. 59, Moscow 119333, Russian Federation.
Jazyk: angličtina
Zdroj: Acta crystallographica. Section D, Structural biology [Acta Crystallogr D Struct Biol] 2017 Jul 01; Vol. 73 (Pt 7), pp. 591-599. Date of Electronic Publication: 2017 Jun 28.
DOI: 10.1107/S2059798317007422
Abstrakt: Solutions of lysozyme in heavy water were studied by small-angle neutron scattering (SANS) at concentrations of 40, 20 and 10 mg ml -1 with and without the addition of precipitant, and at temperatures of 10, 20 and 30°C. In addition to the expected protein monomers, dimeric and octameric species were identified in solutions at the maximum concentration and close to the optimal conditions for crystallization. An optimal temperature for octamer formation was identified and both deviation from this temperature and a reduction in protein concentration led to a significant decrease in the volume fractions of octamers detected. In the absence of precipitant, only monomers and a minor fraction of dimers are present in solution.
Databáze: MEDLINE
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