Effects of peritoneal macrophage autophagy on the immune function of sepsis mice.

Autor:	Zhang DL; The First Affiliated Hospital, School of Medicine, Shihezi UniversityShihezi 832002, Xinjiang, China., Zhang SW; School of Basic Medical Sciences, Nanjing Medical UniversityNanjing 211166, Jiangsu Province, China., Cheng QH; The First Affiliated Hospital, School of Medicine, Shihezi UniversityShihezi 832002, Xinjiang, China., Wu F; The Key Laboratory of Xinjiang Endemic and Ethnic Diseases, Department of Pathophysiology, School of Medicine, Shihezi University, Collaborative Innovation Center of High Incidence of Zoonoses Communicable Disease Prevention in the Western RegionShihezi 832002, Xinjiang, China., Wu JD; The Key Laboratory of Xinjiang Endemic and Ethnic Diseases, Department of Pathophysiology, School of Medicine, Shihezi University, Collaborative Innovation Center of High Incidence of Zoonoses Communicable Disease Prevention in the Western RegionShihezi 832002, Xinjiang, China., Zhang J; The First Affiliated Hospital, School of Medicine, Shihezi UniversityShihezi 832002, Xinjiang, China., Dong JT; The First Affiliated Hospital, School of Medicine, Shihezi UniversityShihezi 832002, Xinjiang, China., Zhu HY; The Key Laboratory of Xinjiang Endemic and Ethnic Diseases, Department of Pathophysiology, School of Medicine, Shihezi University, Collaborative Innovation Center of High Incidence of Zoonoses Communicable Disease Prevention in the Western RegionShihezi 832002, Xinjiang, China., Zhang S; The Key Laboratory of Xinjiang Endemic and Ethnic Diseases, Department of Pathophysiology, School of Medicine, Shihezi University, Collaborative Innovation Center of High Incidence of Zoonoses Communicable Disease Prevention in the Western RegionShihezi 832002, Xinjiang, China., Wu QQ; The First Affiliated Hospital, School of Medicine, Shihezi UniversityShihezi 832002, Xinjiang, China., Zhang WJ; The First Affiliated Hospital, School of Medicine, Shihezi UniversityShihezi 832002, Xinjiang, China.; The Key Laboratory of Xinjiang Endemic and Ethnic Diseases, Department of Pathophysiology, School of Medicine, Shihezi University, Collaborative Innovation Center of High Incidence of Zoonoses Communicable Disease Prevention in the Western RegionShihezi 832002, Xinjiang, China.
Jazyk:	angličtina
Zdroj:	American journal of clinical and experimental immunology [Am J Clin Exp Immunol] 2017 Jun 15; Vol. 6 (4), pp. 52-59. Date of Electronic Publication: 2017 Jun 15 (Print Publication: 2017).
Abstrakt:	Objective: To investigate the effect of peritoneal macrophage autophagy on immune function in sepsis mice. Methods: Seventy-two male BALB/C mice were intraperitoneally injected with LPS to induce sepsis. The mice were randomly divided into six groups: LPS+2 h, LPS+6 h, LPS+12 h, LPS+24 h and LPS+36 h. LPS with a dose of 10 mg/kg was intraperitoneally injected into the abdominal cavity of the sepsis mice, and the control group was injected with the same dose of saline. ELISA was used to detect the concentrations of inflammatory factors IL-2, IL-10 and TNF-α in the peripheral blood, and the CD4+T/CD8+T ratio in the peripheral blood was detected by flow cytometry. The expression levels of LC3II and Beclin-1/beta-action in the mouse macrophages were measured using Western blot to determine the level of autophagy. Results: The expression levels of LC3II and Beclin-1 were significantly higher in the peritoneal macrophages of the mice from the LPS+2 h group than in those of the mice from the normal group (P<0.05). Meanwhile, these levels continuously declined in the LPS+6 h, LPS+12 h, LPS+24 h and LPS+36 h groups (P<0.05). The peripheral blood CD4+T/CD8+T cell ratio was significantly higher in the LPS+2 h and LPS+6 h groups than in the normal group (P<0.05). The ratio peaked at 6 h and then continuously declined (P<0.05). Furthermore, the concentrations of IL-2 and Tnf-α were significantly higher in the peripheral blood serum of the LPS+2 h, LPS+6 h and LPS+12 h groups than in those of the normal group (P<0.05). The peak was observed at 12 h followed by a continuous decline in the LPS+24 h and 3 LPS+6 h groups (P<0.05). The peripheral serum IL-10 concentration was significantly higher in the LPS+2 h, LPS+6 h, LPS+12 h, LPS+24 h and LPS+36 h groups than in the normal group (P<0.05). In the LPS+6 h, LPS+12 h, LPS+24 h and LPS+36 h groups, the peritoneal macrophages LC3II, Beclin-1 and peripheral serum CD+4T/CD+8T ratio correlation index R 2 =0.716 (P=0.043), R 2 =0.954 (P=0.023). Conclusion: Autophagy in peritoneal macrophages plays an important role in the immune function of sepsis mice. In addition, the autophagy of peritoneal macrophages and the immune function of sepsis mice are strongly correlated. Furthermore, macrophage autophagy plays an important role in the immune function changes in sepsis mice, and the underlying mechanism may be involved in inflammation and macrophage antigen presentation by regulating the secretion of inflammatory cytokines and lymphocyte apoptosis antagonism. Competing Interests: None.
Databáze:	MEDLINE
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