| Autor: |
Ko EY; Jeju Center, Korea Basic Science Institute (KBSI), Jeju 690-140, Republic of Korea., Cho SH; Jeju Center, Korea Basic Science Institute (KBSI), Jeju 690-140, Republic of Korea.; Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea., Kang K; BKSU Lnc. Jeju , 63243, Republic of Korea., Kim G; BKSU Lnc. Jeju , 63243, Republic of Korea., Lee JH; Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea., Jeon YJ; Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea., Kim D; Jeju Center, Korea Basic Science Institute (KBSI), Jeju 690-140, Republic of Korea., Ahn G; Department of Marine Bio-food science, College of Fisheries and Ocean Sciences, Chonnam National University, 500-749, Republic of Korea., Kim KN; Chuncheon Center, Korea Basic Science Institute (KBSI), Chuncheon 24341, Republic of Korea. |
| Abstrakt: |
The present study was performed to investigate the anti-inflammatory activity of Tetragonia tetragonoides hydrosols (TTH) and its underlying mechanism in lipopolysaccharide (LPS)-induced RAW 264.7 cells. Gas chromatography (GC) coupled with mass spectrometry and retention index calculations showed that TTH were mainly composed of tetratetracontane (29.5 %), nonacosane (27.6 %), and oleamide (17.1 %). TTH significantly decreased the production of nitric oxide (NO), prostaglandin E2 (PGE 2 ), interleukin (IL)-6, and IL-1β in LPS-stimulated RAW 264.7 cells. Consistent with these observations, TTH treatment decreased the protein expression levels of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2). The molecular mechanism of its anti-inflammatory activity was found to be associated with inhibition of nuclear factor-kappa B (NF-κB) phosphorylation and nuclear translocation of NF-κB 65. Furthermore, TTH markedly suppressed the LPS-induced phosphorylation of mitogen-activated protein kinases (MAPKs). Taken together, these data indicate that TTH exerts an anti-inflammatory activity by inhibiting the NF-κB and MAPK signaling pathways in LPS-stimulated RAW 264.7 cells. |
