Phototoxic assessment of a sunscreen formulation and its excipients: An in vivo and in vitro study.

Autor:	Hossy BH; Programa de Pós-Graduação em Clínica Médica, Faculdade de Medicina - Serviço de Dermatologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil., da Costa Leitão AA; Laboratório de Radiobiologia Molecular, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil., Dos Santos EP; Departamento de Medicamentos, Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil., Matsuda M; Laboratório de Investigação em Oftalmologia (LIM-33), Universidade de São Paulo, Faculdade de Medicina, São Paulo, Brazil., Rezende LB; Laboratório de Microbiologia e Avaliação Genotóxica, Departamento de Análises Clínicas e Toxicológicas, Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil; Programa de Biologia Celular e do Desenvolvimento, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro Federal, Rio de Janeiro, Brazil., Rurr JSC; Laboratório de Radiobiologia Molecular, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil., Pinto AV; Instituto Nacional de Controle de Qualidade em Saúde, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil., Ramos-E-Silva M; Programa de Pós-Graduação em Clínica Médica, Faculdade de Medicina - Serviço de Dermatologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil., de Pádula M; Laboratório de Microbiologia e Avaliação Genotóxica, Departamento de Análises Clínicas e Toxicológicas, Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil., de Oliveira Miguel NC; Programa de Biologia Celular e do Desenvolvimento, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro Federal, Rio de Janeiro, Brazil. Electronic address: miguelnco@histo.ufrj.br.
Jazyk:	angličtina
Zdroj:	Journal of photochemistry and photobiology. B, Biology [J Photochem Photobiol B] 2017 Aug; Vol. 173, pp. 545-550. Date of Electronic Publication: 2017 Jun 29.
DOI:	10.1016/j.jphotobiol.2017.06.043
Abstrakt:	Background: Cosmetic preservatives are used to protect cosmetic formulations and improve its shelf-life. However, these substances may exert phototoxic effects when used under sunlight. Objective: To assess safety, efficacy and putative phototoxic effects of a sunscreen formulation SPF 30 and its excipients. Materials/methods: Irradiation was performed with solar simulated light (SSL) and the sunscreen from the School of Pharmacy/UFRJ/Brazil. We used albino hairless mice in different groups (control (G1), only irradiated (G2), sunscreen plus irradiation (G3) and vehicle plus irradiation (G4) for morphological assessment and immunefluorescence detection to OKL38. In vitro analyses were with a Saccharomyces cerevisiae (SC) strain plus SSL in the presence of methylparaben, propylparaben, imidazolidinyl urea, aminomethyl propanol and their association. Results: G3 and G4 displayed photosensitization leading to thickening of the epidermis and increased dermal cellularity. G4 displayed strong OKL38 labeling when compared with other groups. Aminomethyl propanol, methylparaben and propylparaben are endowed with phototoxic activity against SC. Propylparaben displayed the highest phototoxic effect, followed by excipients association. Conclusions: The sunscreen's vehicle is endowed with phototoxic activity. Propylparaben was the most phototoxic agent, increasing the overall phototoxicity of excipient association, pointing to a critical concern regarding vehicle associations intended to cosmetic purposes. (Copyright © 2017 Elsevier B.V. All rights reserved.)
Databáze:	MEDLINE
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