| Autor: |
Saucedo-Espinosa MA; Microscale Bioseparations Laboratory, Rochester Institute of Technology , Rochester, New York 14623, United States., Lapizco-Encinas BH; Microscale Bioseparations Laboratory, Rochester Institute of Technology , Rochester, New York 14623, United States. |
| Jazyk: |
angličtina |
| Zdroj: |
Analytical chemistry [Anal Chem] 2017 Aug 15; Vol. 89 (16), pp. 8459-8467. Date of Electronic Publication: 2017 Jul 25. |
| DOI: |
10.1021/acs.analchem.7b02008 |
| Abstrakt: |
Dielectrophoresis (DEP) is the motion of particles under the influence of a nonuniform electric field. In insulator-based dielectrophoresis (iDEP), the required nonuniform electric fields are generated with insulating structures embedded in a microchannel. These structures distort the electric field distribution when an electric potential is applied. This contribution presents an experimental characterization of the electrokinetic (EK) and DEP velocities of a set of target particles, under DC potentials, when additional innocuous particles are used as fillers. Streak-based particle velocimetry in a tapered channel was used to assess particle motion. Filler particles of various sizes were added at different volume fractions (ϕ) to suspending media containing the target particles/cells. The presence of the filler particles resulted in electric field distortions and dissimilar particle behaviors caused by particle-particle interactions. These particle mutual interactions were exploited to improve the enrichment of low-abundance yeast cells in an iDEP channel. It was shown that the smallest studied filler particles (500 nm) have the potential to aid the enrichment of low-abundance yeast cells when filler volume fractions ∼1 × 10 -5 v/v are used. Enrichment factors of ∼115 were achieved by applying electric potentials as low as 500 V. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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