The Potential of Gelam Honey in Promoting the Proliferative Phase of Corneal Reepithelialization.

Autor:	Azmi MF; Department of Anatomy, Universiti Kebangsaan Malaysia Medical Centre, Bandar Tun Razak, Kuala Lumpur, Malaysia; Discipline of Anatomy, Faculty of Medicine, Universiti Teknologi MARA, Jalan Hospital, Selangor, Malaysia., Ghafar NA; Department of Anatomy, Universiti Kebangsaan Malaysia Medical Centre, Bandar Tun Razak, Kuala Lumpur, Malaysia., Hamzah JC; Department of Ophthalmology, Universiti Kebangsaan Malaysia Medical Centre., Luan NS; Department of Physiology, Universiti Kebangsaan Malaysia Medical Centre., Hui CK; Department of Physiology, Universiti Kebangsaan Malaysia Medical Centre.
Jazyk:	angličtina
Zdroj:	Wounds : a compendium of clinical research and practice [Wounds] 2017 Nov; Vol. 29 (11), pp. 327-332. Date of Electronic Publication: 2017 Jun 28.
Abstrakt:	Objective: The aim of this study is to investigate the potential bene ts of Gelam honey (GH) in promoting proliferation of ex vivo cor- neal epithelial cells (CECs) and its effects on the phenotypical features. Materials and Methods: Corneal epithelial cells were isolated from the corneas of rabbits (n = 6). The optimal dose of GH for CEC proliferation in both basal medium (BM) and cornea medium (CM) was determined via MTT (3-[4, 5-dimethyl thiazolyl-2]-2, 5-diphenyl tetrazolium bro- mide) assay. Morphology, gene and protein expressions, and cell cycle analysis of CECs were evaluated via phase contrast microscopy, real- time polymerase chain reaction, immunocytochemistry, and ow cytom- etry, respectively. Results: Corneal epithelial cells cultured in 0.0015% GH-supplemented media (BM + 0.0015% GH; CM + 0.0015% GH) demonstrated optimal proliferative capacity with normal polygonal- shaped morphology. Gelam honey potentiates cytokeratin 3 (CK3) gene expression in accordance with the cytoplasmic CK3 protein expression while retaining normal cell cycle of CECs. Conclusion: Culture media treated with 0.0015% GH increased CEC proliferation while preserving its phenotypical features. This study demonstrated the potential devel- opment of GH-based topical treatment for super cial corneal injury.
Databáze:	MEDLINE
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