Increased FNDC5 is associated with insulin resistance in high fat-fed mice.

Autor: Guilford BL; Department of Applied Health, Southern Illinois University Edwardsville, Edwardsville, Illinois bguilfo@siue.edu., Parson JC; Department of Applied Health, Southern Illinois University Edwardsville, Edwardsville, Illinois., Grote CW; Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, Kansas., Vick SN; Department of Pharmacy Practice, Southern Illinois University Edwardsville, Edwardsville, Illinois., Ryals JM; Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, Kansas., Wright DE; Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, Kansas.
Jazyk: angličtina
Zdroj: Physiological reports [Physiol Rep] 2017 Jul; Vol. 5 (13).
DOI: 10.14814/phy2.13319
Abstrakt: FNDC5/irisin, has recently been identified as a novel protein that stimulates the "browning" of white adipose by inducing thermogenesis via increased uncoupling protein 1 (UCP1). We tested the hypothesis that high fat diet-induced prediabetic mice would exhibit increased FNDC5 and this effect would be attenuated by chronic exercise. C57BL/6 mice were randomized into three groups for the 4 week intervention: Standard diet (Std, n  =   12), High fat diet (HF, n  =   14), or High fat diet and free access to a running wheel (HFEX, n  =   14). Body weight, glucose, insulin, and the homeostatic model assessment of insulin resistance (HOMA-IR) were greater in HF compared to Std and HFEX after the 4 week intervention. In support of our hypothesis, FNDC5 was higher in HF in both skeletal muscle and adipose compared to Std and was lower in adipose only in HFEX compared to HF mice. Following the same pattern, PGC-1 α was significantly higher in HF compared to Std in skeletal muscle and significantly lower in HFEX compared to HF in adipose. UCP1 was significantly lower in HFEX versus Std (in skeletal muscle) and versus HF (in adipose). HOMA-IR was significantly correlated with FNDC5 protein levels in adipose. Increased FNDC5 in adipose and skeletal muscle may be a compensatory mechanism to offset high fat diet-induced weight gain and insulin resistance by increasing energy expenditure.
(© 2017 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society.)
Databáze: MEDLINE
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