Maintenance of head and neck tumor on-chip: gateway to personalized treatment?

Autor:	Bower R; School of Life Sciences, The University of Hull, Cottingham Road, Hull, HU6 7RX, UK.; School of Life Sciences, The University of Hull, Cottingham Road, Hull, HU6 7RX, UK., Green VL; School of Life Sciences, The University of Hull, Cottingham Road, Hull, HU6 7RX, UK.; School of Life Sciences, The University of Hull, Cottingham Road, Hull, HU6 7RX, UK., Kuvshinova E; Department of Chemical & Biological Engineering, The University of Sheffield, Mappin Street, Sheffield, S1 3JD, UK.; Department of Chemical & Biological Engineering, The University of Sheffield, Mappin Street, Sheffield, S1 3JD, UK., Kuvshinov D; School of Engineering & Computer Science, The University of Hull, Cottingham Road, Hull, HU6 7RX, UK.; School of Engineering & Computer Science, The University of Hull, Cottingham Road, Hull, HU6 7RX, UK., Karsai L; Department of Cellular Pathology, Hull Royal Infirmary, Anlaby Road, Hull, HU3 2JZ, UK.; Department of Cellular Pathology, Hull Royal Infirmary, Anlaby Road, Hull, HU3 2JZ, UK., Crank ST; Department of Oral & Maxillofacial Surgery, Hull Royal Infirmary, Anlaby Road, Hull, HU3 2JZ, UK.; Department of Oral & Maxillofacial Surgery, Hull Royal Infirmary, Anlaby Road, Hull, HU3 2JZ, UK., Stafford ND; Castle Hill Hospital, University of Hull, Daisy Building, Cottingham, HU16 5JQ, UK.; Castle Hill Hospital, University of Hull, Daisy Building, Cottingham, HU16 5JQ, UK., Greenman J; School of Life Sciences, The University of Hull, Cottingham Road, Hull, HU6 7RX, UK.; School of Life Sciences, The University of Hull, Cottingham Road, Hull, HU6 7RX, UK.
Jazyk:	angličtina
Zdroj:	Future science OA [Future Sci OA] 2017 Mar 07; Vol. 3 (2), pp. FSO174. Date of Electronic Publication: 2017 Mar 07 (Print Publication: 2017).
DOI:	10.4155/fsoa-2016-0089
Abstrakt:	Aim: Head and neck squamous cell carcinomas (HNSCC) are solid tumors with low overall survival (40-60%). In a move toward personalized medicine, maintenance of tumor biopsies in microfluidic tissue culture devices is being developed. Methodology/results: HNSCC (n = 15) was dissected (5-10 mg) and either analyzed immediately or cultured in a microfluidic device (37°C) for 48 h. No difference was observed in morphology between pre- and postculture specimens. Dissociated samples were analyzed using trypan blue exclusion (viability), propidium iodide flow cytometry (death) and MTS assay (proliferation) with no significant difference observed highlighting tissue maintenance. Computational fluid dynamics showed laminar flow within the system. Conclusion: The microfluidic culture system successfully maintained HNSCC for 48 h, the culture system will allow testing of different treatment modalities with response monitoring. Competing Interests: Financial & competing interests disclosure The authors have no relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript. This includes employment, consultancies, honoraria, stock ownership or options, expert testimony, grants or patents received or pending, or royalties. No writing assistance was utilized in the production of this manuscript.
Databáze:	MEDLINE
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