An RNA Recognition Motif-Containing Protein Functions in Meiotic Silencing by Unpaired DNA.

Autor: Samarajeewa DA; School of Biological Sciences, Illinois State University, Normal, Illinois 61790., Manitchotpisit P; School of Biological Sciences, Illinois State University, Normal, Illinois 61790., Henderson M; School of Biological Sciences, Illinois State University, Normal, Illinois 61790., Xiao H; Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211., Rehard DG; Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211., Edwards KA; School of Biological Sciences, Illinois State University, Normal, Illinois 61790., Shiu PKT; Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211., Hammond TM; School of Biological Sciences, Illinois State University, Normal, Illinois 61790 tmhammo@ilstu.edu.
Jazyk: angličtina
Zdroj: G3 (Bethesda, Md.) [G3 (Bethesda)] 2017 Aug 07; Vol. 7 (8), pp. 2871-2882. Date of Electronic Publication: 2017 Aug 07.
DOI: 10.1534/g3.117.041848
Abstrakt: Meiotic silencing by unpaired DNA (MSUD) is a biological process that searches pairs of homologous chromosomes (homologs) for segments of DNA that are unpaired. Genes found within unpaired segments are silenced for the duration of meiosis. In this report, we describe the identification and characterization of Neurospora crassa sad-7 , a gene that encodes a protein with an RNA recognition motif (RRM). Orthologs of sad-7 are found in a wide range of ascomycete fungi. In N. crassa , sad-7 is required for a fully efficient MSUD response to unpaired genes. Additionally, at least one parent must have a functional sad-7 allele for a cross to produce ascospores. Although sad-7 -null crosses are barren, sad-7 Δ strains grow at a wild-type (wt) rate and appear normal under vegetative growth conditions. With respect to expression, sad-7 is transcribed at baseline levels in early vegetative cultures, at slightly higher levels in mating-competent cultures, and is at its highest level during mating. These findings suggest that SAD-7 is specific to mating-competent and sexual cultures. Although the role of SAD-7 in MSUD remains elusive, green fluorescent protein (GFP)-based tagging studies place SAD-7 within nuclei, perinuclear regions, and cytoplasmic foci of meiotic cells. This localization pattern is unique among known MSUD proteins and raises the possibility that SAD-7 coordinates nuclear, perinuclear, and cytoplasmic aspects of MSUD.
(Copyright © 2017 Samarajeewa et al.)
Databáze: MEDLINE
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