Bioinspired coupled helical coils for soft tissue engineering of tubular structures - Improved mechanical behavior of tubular collagen type I templates.

Autor: Janke HP; Department of Urology, Radboud Institute for Molecular Life Science, Radboud University Medical Center, Geert Grooteplein 28, 6525 GE Nijmegen, The Netherlands. Electronic address: heinzpeter.janke@radboudumc.nl., Bohlin J; Department of Chemistry - Division of Polymer Chemistry, Ångström Laboratory, A Science for Life Laboratory, Uppsala University, Lägerhyddsvägen 1, 751 21 Uppsala, Sweden. Electronic address: jan.bohlin@kemi.uu.se., Lomme RMLM; Department of Surgery, Radboud University Medical Center, Geert Grooteplein 26, 6525 GE Nijmegen, The Netherlands. Electronic address: roger.lomme@radboudumc.nl., Mihaila SM; Department of Urology, Radboud Institute for Molecular Life Science, Radboud University Medical Center, Geert Grooteplein 28, 6525 GE Nijmegen, The Netherlands. Electronic address: silvia.mihaila@radboudumc.nl., Hilborn J; Department of Chemistry - Division of Polymer Chemistry, Ångström Laboratory, A Science for Life Laboratory, Uppsala University, Lägerhyddsvägen 1, 751 21 Uppsala, Sweden. Electronic address: jons.hilborn@kemi.uu.se., Feitz WFJ; Department of Urology, Radboud Institute for Molecular Life Science, Radboud University Medical Center, Geert Grooteplein 28, 6525 GE Nijmegen, The Netherlands; Radboudumc Amalia Children's Hospital, Geert Grooteplein-Zuid 10, 6525 GA Nijmegen, The Netherlands. Electronic address: wout.feitz@radboudumc.nl., Oosterwijk E; Department of Urology, Radboud Institute for Molecular Life Science, Radboud University Medical Center, Geert Grooteplein 28, 6525 GE Nijmegen, The Netherlands. Electronic address: egbert.oosterwijk@radboudumc.nl.
Jazyk: angličtina
Zdroj: Acta biomaterialia [Acta Biomater] 2017 Sep 01; Vol. 59, pp. 234-242. Date of Electronic Publication: 2017 Jun 27.
DOI: 10.1016/j.actbio.2017.06.038
Abstrakt: The design of constructs for tubular tissue engineering is challenging. Most biomaterials need to be reinforced with supporting structures such as knittings, meshes or electrospun material to comply with the mechanical demands of native tissues. In this study, coupled helical coils (CHCs) were manufactured to mimic collagen fiber orientation as found in nature. Monofilaments of different commercially available biodegradable polymers were wound and subsequently fused, resulting in right-handed and left-handed polymer helices fused together in joints where the filaments cross. CHCs of different polymer composition were tested to determine the tensile strength, strain recovery, hysteresis, compressive strength and degradation of CHCs of different composition. Subsequently, seamless and stable hybrid constructs consisting of PDSII® USP 2-0 CHCs embedded in porous collagen type I were produced. Compared to collagen alone, this hybrid showed superior strain recovery (93.5±0.9% vs 71.1±12.6% in longitudinal direction; 87.1±6.6% vs 57.2±4.6% in circumferential direction) and hysteresis (18.9±2.7% vs 51.1±12.0% in longitudinal direction; 11.5±4.6% vs 46.3±6.3% in circumferential direction). Furthermore, this hybrid construct showed an improved Young's modulus in both longitudinal (0.5±0.1MPavs 0.2±0.1MPa; 2.5-fold) and circumferential (1.65±0.07MPavs (2.9±0.3)×10 -2 MPa; 57-fold) direction, respectively, compared to templates created from collagen alone. Moreover, hybrid template characteristics could be modified by changing the CHC composition and CHCs were produced showing a mechanical behavior similar to the native ureter. CHC-enforced templates, which are easily tunable to meet different demands may be promising for tubular tissue engineering.
Statement of Significance: Most tubular constructs lack sufficient strength and tunability to comply with the mechanical demands of native tissues. Therefore, we embedded coupled helical coils (CHCs) produced from biodegradable polymers - to mimic collagen fiber orientation as found in nature - in collagen type I sponges. We show that the mechanical behavior of CHCs is very similar to native tissue and strengths structurally weak tubular constructs. The production procedure is relatively easy, reproducible and mechanical features can be controlled to meet different mechanical demands. This is promising in template manufacture, hence offering new opportunities in tissue engineering of tubular organs and preventing graft failure.
(Copyright © 2017 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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