Evaluation of Biomarkers in Egyptian Patients with Different Grades of Nonalcoholic Fatty Liver Disease.

Autor:	Borai IH; Biochemistry Department, Faculty of Science, Ain Shams University, Cairo, Egypt., Shaker Y; Biochemistry Department, National Research Centre, Giza, Egypt., Kamal MM; Biochemistry Department, Faculty of Science, Ain Shams University, Cairo, Egypt., Ezzat WM; Department of Internal Medicine, National Research Center, Giza, Egypt., Ashour E; Biochemistry Department, National Research Centre, Giza, Egypt., Afify M; Biochemistry Department, National Research Centre, Giza, Egypt., Gouda W; Biochemistry Department, National Research Centre, Giza, Egypt., Elbrashy MM; Biochemistry Department, National Research Centre, Giza, Egypt.
Jazyk:	angličtina
Zdroj:	Journal of clinical and translational hepatology [J Clin Transl Hepatol] 2017 Jun 28; Vol. 5 (2), pp. 109-118. Date of Electronic Publication: 2017 Jun 07.
DOI:	10.14218/JCTH.2017.00004
Abstrakt:	Background and Aims: Nonalcoholic fatty liver disease (NAFLD) is a silent disease; its spectrum includes simple steatosis, nonalcoholic steatohepatitis and fibrosis. Pro- and anti-inflammatory cytokines play roles in the pathogenesis of NAFLD and insulin resistance (IR). Moreover, plasma cell antigen-1 (PC-1) is related to IR and associated with NAFLD progression. Therefore, we aimed to detect biomarkers, ultrasonographic and anthropometric findings capable of differentiating NAFLD grades, since most previous investigators were concerned more with NAFLD patients without classifying them into grades. Methods: A total of 87 NAFLD patients (31 with grade 1 (mild NAFLD), 26 with grade 2 (moderate NAFLD) and 30 with grade 3 (severe NAFLD) were included in the study, in addition to 47 controls (grade 0). All subjects underwent ultrasonographic examination for NAFLD diagnosis. Serum interleukin-10 (IL-10), plasma interleukin-18 (IL-18) and plasma PC-1 levels were determined using enzyme-linked immunosorbent assay. Results: Homoeostasis model assessment (HOMA)-IR was higher in different NAFLD grades than in controls. Ultrasonographic and anthropometric findings and lipid profile indices (except for high-density lipoprotein cholesterol, which was decreased) were increased with NAFLD progression. Grade 3 patients showed significant increase in levels of IL-18 and significant decrease in IL-10 and PC-1 levels when compared to grade 1 patients. Conclusion: Anthropometric and ultrasonographic findings were valuable in differentiating NAFLD grades. IR is very important in NAFLD pathogenesis. IL-18, HOMA-index and PC-1 levels could be used to differentiate between NAFLD grades, together with other measurements. Competing Interests: The authors have no conflict of interests related to this publication.
Databáze:	MEDLINE
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