Antioxidant activities and phytochemical constituents of Antidesma thwaitesianum Müll. Arg. leaf extracts.
Autor: | Dechayont B; Department of Applied Thai Traditional Medicine, Faculty of Medicine, Thammasat University, Pathumthani 12120, Thailand., Itharat A; Department of Applied Thai Traditional Medicine, Faculty of Medicine, Thammasat University, Pathumthani 12120, Thailand.; Center of Excellence in Applied Thai Traditional Medicine Research, Thammasat University, Pathumthani 12120, Thailand., Phuaklee P; Department of Applied Thai Traditional Medicine, Faculty of Medicine, Thammasat University, Pathumthani 12120, Thailand., Chunthorng-Orn J; Department of Applied Thai Traditional Medicine, Faculty of Medicine, Thammasat University, Pathumthani 12120, Thailand., Juckmeta T; Department of Applied Thai Traditional Medicine, Faculty of Medicine, Thammasat University, Pathumthani 12120, Thailand., Prommee N; Division of Applied Thai Traditional Medicine, Faculty of Public Health, Naresuan University, Phitsanulok 65000, Thailand., Nuengchamnong N; Science Laboratory Centre, Faculty of Science, Naresuan University, Phitsanulok 65000, Thailand., Hansakul P; Center of Excellence in Applied Thai Traditional Medicine Research, Thammasat University, Pathumthani 12120, Thailand.; Department of Preclinical Science, Faculty of Medicine, Thammasat University, Pathumthani 12120, Thailand. |
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Jazyk: | angličtina |
Zdroj: | Journal of integrative medicine [J Integr Med] 2017 Jul; Vol. 15 (4), pp. 310-319. |
DOI: | 10.1016/S2095-4964(17)60334-0 |
Abstrakt: | Objective: To investigate the antioxidant activities as well as phytochemical constituents of Antidesma thwaitesianum Müll. Arg. leaf extracts. Methods: The leaves of A. thwaitesianum were extracted using three different methods: blending with distilled water, maceration with ethanol and decoction. The chemical antioxidant activity of the plant leaf extracts was evaluated using 2,2-diphenyl-1-picryhydrazyl (DPPH) radical and 2,2'-azinobis(3-ethylbenzo-thiazoline-6-sulfonic acid) diammonium salt (ABTS⁺) radical scavenging assays, as well as the ferric reducing antioxidant power assay. Cellular antioxidant activity was determined by superoxide and nitric oxide scavenging assays. The cytotoxicity of the leaf extracts in RAW 264.7 and differentiated HL-60 cells was tested in parallel using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide and 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium assays, respectively. The total phenolic and flavonoid contents were also assessed by spectrophotometric analysis. Phytochemical constituents of the most potent extract were investigated by liquid chromatography with an electrospray ionization quadrupole time-of-flight mass spectrometer (LC-ESI-QTOF-MS/MS). Results: The ethanolic (ME) and decoction (LW) extracts of dried leaves had the highest chemical scavenging activity against DPPH and ABTS⁺ free radicals with half maximal effective concentration (EC Conclusion: This is the first report describing potent chemical and cellular antioxidant effects of the ethanolic leaf extract of A. thwaitesianum. The extract contained high total phenolic and flavonoid contents. LC-ESI-QTOF-MS/MS analysis further revealed an abundance of caffeic acid derivatives and flavonoids. These data support its potential use as dietary supplements in oxidative stress prevention. |
Databáze: | MEDLINE |
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