Adipose Tissue Graft Improves Early but not Late Stages of Nerve Regeneration.

Autor:	Bloancă V; Department of Plastic Surgery, Victor Babes University of Medicine and Pharmacy, Timişoara, Romania., Ceauşu AR; Department of Microscopic Morphology/Histology, Victor Babes University of Medicine and Pharmacy, Timişoara, Romania., Jitariu AA; Department of Microscopic Morphology/Histology, Victor Babes University of Medicine and Pharmacy, Timişoara, Romania., Barmayoun A; Angiogenesis Research Center, Victor Babes University of Medicine and Pharmacy, Timişoara, Romania., Moş R; Angiogenesis Research Center, Victor Babes University of Medicine and Pharmacy, Timişoara, Romania., Crăiniceanu Z; Department of Plastic Surgery, Victor Babes University of Medicine and Pharmacy, Timişoara, Romania zcrainiceanu@gmail.com., Bratu T; Department of Plastic Surgery, Victor Babes University of Medicine and Pharmacy, Timişoara, Romania.
Jazyk:	angličtina
Zdroj:	In vivo (Athens, Greece) [In Vivo] 2017 Jul-Aug; Vol. 31 (4), pp. 649-655.
DOI:	10.21873/invivo.11107
Abstrakt:	Aim: The aim of the study was to assess the effect of autologous fat graft on nerve regeneration by means of immunohistochemistry. Materials and Methods: The rat sciatic nerve was used; complete transection followed by primary neurorrhaphy was performed on both hind legs, on the left side a processed fat graft was applied, surrounding the nerve. Nerve biopsies were collected and immunohistochemical procedures were performed for glial fibrillary acidic protein (GFAP) and for neurofilament-associated protein(NFAP). Results: At 4 weeks, GFAP-positive cells were observed in the connective tissue formed between the two nerve endings on the left side only. At 10 weeks, GFAP-positive structures were present and exhibited a tendency to become linear on both sides, with an increased density on the left. NFAP-positive expression was present in the left treated limb with a disorganized pattern. Conclusion: Adipose tissue led to the stimulation of GFAP-positive Schwann cells, which could have a positive impact on nerve regeneration in the clinical setting. (Copyright© 2017, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.)
Databáze:	MEDLINE
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