B10 Cells Alleviate Periodontal Bone Loss in Experimental Periodontitis.

Autor:	Wang Y; The Forsyth Institute, Department of Immunology and Infectious Diseases, Cambridge, Massachusetts, USA.; Shanghai 9th People's Hospital Affiliated Shanghai JiaoTong University School of Medicine, Department of Stomatology, Shanghai, China., Yu X; The Forsyth Institute, Department of Immunology and Infectious Diseases, Cambridge, Massachusetts, USA.; Peking University School and Hospital of Stomatology, Department of Periodontology, Beijing, China., Lin J; The Forsyth Institute, Department of Immunology and Infectious Diseases, Cambridge, Massachusetts, USA.; The Fourth Hospital of Harbin Medical University, Department of Stomatology, Harbin, China., Hu Y; The Forsyth Institute, Department of Immunology and Infectious Diseases, Cambridge, Massachusetts, USA., Zhao Q; The Forsyth Institute, Department of Immunology and Infectious Diseases, Cambridge, Massachusetts, USA.; Beijing ChaoYang Hospital Affiliated to Capital Medical University, Department of Stomatology, Beijing, China., Kawai T; The Forsyth Institute, Department of Immunology and Infectious Diseases, Cambridge, Massachusetts, USA., Taubman MA; The Forsyth Institute, Department of Immunology and Infectious Diseases, Cambridge, Massachusetts, USA., Han X; The Forsyth Institute, Department of Immunology and Infectious Diseases, Cambridge, Massachusetts, USA Xhan@forsyth.org.
Jazyk:	angličtina
Zdroj:	Infection and immunity [Infect Immun] 2017 Aug 18; Vol. 85 (9). Date of Electronic Publication: 2017 Aug 18 (Print Publication: 2017).
DOI:	10.1128/IAI.00335-17
Abstrakt:	B10 cells can regulate inflammatory responses in innate immunity. Toll-like receptors (TLRs) play an important role in B cell-mediated immune responses in periodontal disease. This study aimed to determine the effects of TLR-activated B10 cells on periodontal bone loss in experimental periodontitis. Spleen B cells isolated from C57BL/6J mice were cultured with Porphyromonas gingivalis lipopolysaccharide (LPS) and cytosine-phospho-guanine (CpG) oligodeoxynucleotides for 48 h. B10-enriched CD1d hi CD5 + B cells were sorted by flow cytometry and were adoptively transferred to recipient mice through tail vein injection. At the same time, P. gingivalis -soaked ligatures were placed subgingivally around the maxillary second molars and remained there for 2 weeks before the mice were euthanized. Interleukin-10 (IL-10) production and the percentage of CD1d hi CD5 + B cells were significantly increased with treatment with P. gingivalis LPS plus CpG compared to those in mice treated with P. gingivalis LPS or CpG alone. Mice with CD1d hi CD5 + B cell transfer demonstrated reduced periodontal bone loss compared to the no-transfer group and the group with CD1d lo CD5 - B cell transfer. Gingival IL-10 mRNA expression was significantly increased, whereas expressions of receptor activator of NF-κB ligand (RANKL)/osteoprotegerin (OPG), tumor necrosis factor alpha (TNF-α), and IL-1β were significantly inhibited in the CD1d hi CD5 + B cell transfer group. The percentages of CD19 + IL-10 + cells, CD19 + CD1d hi CD5 + cells, and P. gingivalis -binding CD19 + cells were significantly higher in recovered mononuclear cells from gingival tissues of the CD1d hi CD5 + B cell transfer group than in tissues of the no-transfer group and the CD1d lo CD5 - B cell transfer group. This study indicated that the adoptive transfer of B10 cells alleviated periodontal inflammation and bone loss in experimental periodontitis in mice. (Copyright © 2017 American Society for Microbiology.)
Databáze:	MEDLINE
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