| Autor: |
Bošnjak-Neumüller J; Department of Biology, Faculty of Veterinary Medicine, University of Belgrade, Blvd. oslobodjenja Belgrade, Serbia., Radaković M; Department of Biology, Faculty of Veterinary Medicine, University of Belgrade, Blvd. oslobodjenja Belgrade, Serbia., Djelić N; Department of Biology, Faculty of Veterinary Medicine, University of Belgrade, Blvd. oslobodjenja Belgrade, Serbia., Vuković-Gačić B; Department of Microbiology, Faculty of Biology, University of Belgrade, Studentski trg, Belgrade, Serbia., Stevanović ZD; Department of Crop Science, Faculty of Agriculture, University of Belgrade, Nemanjina Belgrade, Serbia., Kolarević S; Department of Microbiology, Faculty of Biology, University of Belgrade, Studentski trg, Belgrade, Serbia., Mišić D; Institute for Biological Research ''Siniša Stanković'', University of Belgrade, Blvd. Despota Stefana, Belgrade, Serbia., Stanković M; Department of Biology and Ecology, University of Kragujevac, Radoja Domanovića, Kragujevac, Serbia., Knežević-Vukčević J; Department of Microbiology, Faculty of Biology, University of Belgrade, Studentski trg, Belgrade, Serbia., Spremo-Potparević B; Department of Biology and Human Genetics, Institute of Physiology, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe, Belgrade, Serbia., Stanimirović Z; Department of Biology, Faculty of Veterinary Medicine, University of Belgrade, Blvd. oslobodjenja Belgrade, Serbia. |
| Abstrakt: |
The success of antioxidant therapy in hyperthyroidism implies that disease is mediated by oxidative stress, which is known as one of the causing agents of ageing, degenerative diseases, and cancer. The main objective of our study was to determine possible protective effects of methanolic extract of N. rtanjensis in triiodothyronine (T3)-induced DNA breaks of human lymphocytes under in vitro conditions, based upon plant antioxidant capacity related to its phytochemical profile, mainly its polyphenolic complex. The total phenolic and flavonoid content and the antioxidant activity using in vitro 1,1-dyphenyl-2- picrylhydrazyl reagent (DPPH) was determined in methanolic extracts of plant leaves and flowers. The phenolic compound content of 62.73±1.80mg of GaA/g, exhibited solid antioxidant activity (IC 50 = 112.59±0.95μg/ml). The antigenotoxic activity of 0.2, 0.5 and 1.0mg/ml N. rtanjensis methanol extracts mixture with 100µM of T3 was studied in human lymphocytes in vitro using the Comet assay. It is supposed that the antigenotoxicity of N. rtanjensis methanol extracts was caused by high presence of chlorogenic acid, rosmarinic acid and rutin, all known as efficient antioxidant bioactive compounds, which were determined by ultrahigh-pressure liquid chromatograph with MS/MS Mass Spectroscopy (UHPLC/-HESI-MS / MS). |
